响应曲面法优化鼠李糖乳杆菌Ls-8菌株产抑真菌活性物质的条件

Optimization of antagonism activity yield by Lactobacillus casei sudsp. rahamnosus Ls-81 by response surface methodology

在使用Plackett-Burman设计试验的基础上,筛选出发酵时间、乳清粉、蔗糖和乙酸钠为鼠李糖乳杆菌Ls-8菌株发酵产抑真菌活性物质的4个关键影响因子。采用响应曲面法对这4个关键因子进行了优化,根据二次多项式回归方程求解得出,在发酵时间为82h、乳清粉添加量为29g/L、蔗糖添加量为25.5g/L、乙酸钠添加量为10.7g/L时、Ls-8发酵液的抑菌活性可达0.66AU/mL。

Based on the results of the previous Plackett-Burman design, four key factors was chosen, namely fermentation time, whey powder, saccharose and sodium acetate. Response surface methodology was used to optimize the optimal levels of the four important factors. Response surface model computed by guadratic regression methodology show the optimal condition for obtaining 0.66AU antifungal activity per milliliter were： fermentation time 82h, whey powder 29g/L, saccharose 25.5g/L, sodium acetate 10.7g/L.