摘要
目的：建立高效液相色谱测定野木瓜中齐墩果酸含量的方法。方法：采用Shim—packVP—ODS柱（250mm×4．6mm）；流动相为乙腈-1．1％乙酸水（94：6）；流速0．5mL／min；检测波长205nm；柱温为室温；洗脱方式为等度洗脱。结果：齐墩果酸获得良好分离，在0．03—0．15mg／ml范围内线性关系良好，回归方程为Y：3．81424×10^-8x-0．00240239，相关系数r=0．9994，平均加样回收率为100．68％。结论：该方法精密度高，重现性好，简单易行。通过以上方法，测得野木瓜中齐墩果酸平均含量为0．1055％。
To establish an HPLC method to determine the content of oleanolic acid in Stamtonia Chinensis. Method： Oleanolic acid was sperated on an Shim - pack VP - ODScolumn. The mobile phase was acetonitrile - 1. 1% acetic acid water. The flow rate was 0.5ml/min. UV detection wavelength was 205nm. Column temperature was the room temperature. Results ： Oleanolic acid was separated well. The repressive equation is ： y = 3. 81424 × 10 - 8x - 0. 00240239, r = 0.9994. The relationship of injection amounts and peak was linear in the range of 0.03 -0.15mg/ml and the recovery rate was 100. 68%. Conclusion： The method is simple and available,of accuracy and good reproducibility. By this method, the content of oleanolic acid from Stanuntonia Chinensis is 0. 1055%.
出处
《贵州科技工程职业学院学报》
2007年第3期24-27,共4页
GUIZHOU SCIENCE AND TECHNOLOGY PROFESSIONAL COLLEGE
基金
贵州省科技基金（黔科合J字[2005]2017号）.
