普通小麦-冰草衍生后代中抑制成穗新种质的外源物质检测与遗传分析

Characterization of Spike Inhibition Wheat Germplasm Derived from Wheat×Agropyron cristatum

【目的】检测普通小麦Fukuhokomugi(Triticum aestivum L.)-冰草Z559(Agropyron cristatum L.Gaertn.)衍生后代中6个分蘖正常而成穗显著受抑制株系的外源物质,对成穗受抑制性状进行遗传分析。【方法】采用基因组原位杂交(GISH)和微卫星(SSR)技术进行外源物质检测;以成穗受抑制小麦(♀)×京4841(♂)后代的F1与F2单株表型进行遗传分析。【结果】通过GISH和SSR分析,在成穗受抑制株系中检测出2个插入易位和6个SSR易位标记;成穗受抑制材料与京4841的杂交后代F1单株均表现为正常成穗,F2正常成穗植株与成穗显著受抑制植株比值为3﹕1。【结论】有一些冰草的染色体片段被导入普通小麦Fukuhokomugi中,抑制成穗性状的基因是1对隐性基因。

[Objective] The project was conducted to detect exogenous hereditary property and analyze the reason of spike inhibition in spike inhibition plant lines from the progeny lines of the cross between Fukuhokomugi and A. cristatum （L.） Gaertn, （National Gene Bank accession number Z559）. These lines had many natural tillers but only the main stem could come into being. [Method] Detection of A. cristatum chromatin was done by means of genornic in situ hybridization （GISH） and microsatellites （SSR）. Genetic analysis of F1 and F2 plants were derived from the cross of spike inhibition plant lines × Jing 4841 cross. [ Result] GISH showed that these spike inhibition lines were to the translocation lines in which two pairs of chromosomes from wheat and A. cristatum. Total six translational markers were detected by using SSR analysis in them. The F1 plants of the spike inhibition wheat× Jing 4841 were all normal in tillering and ear formation, and F2 progeny plants segregated for normal and abnormal in ear formation performance in a ratio of 3：1. [ Conclusion ] A few chromosome sequences were translated from A. cristatum to Fukuhokomugi and a single recessive gene was responsible for spike inhibition and ear formation.