应用酶法和电极法测定血清钾钠氯的实验对比

Comparison of enzymoanalysis with electroanalysis on the determination of potassium,sodium and chloride

为了解酶法分析K+、Na+、Cl-的特点，应用电极法进行了实验对比，结果二种方法线性范围和平均回收率均符合要求，精密度实验批内和批间CV值均在允许范围，但电极法明显优于酶法，用二种方法测定K+、Na+、Cl-的相关系数分别为0．9767，0．9685和0．9525，且差异不显著（P＞0．05），溶血样品对二种方法测K+均有明显正干扰，高脂血样品对电权法无干扰，而对酸法测Na+、Cl-则有明显负干扰。虽然电极法优于酶法，但酶法的各种实验参数也符合方法学要求。

Enzymoanalysis and electroanalysis were compared in tbe deter. mination of potassium,sodium and chlo-ride. The results revealed that the linearity and the average rate of recovery were both agree with the stan-dard. Electroanalysis has advantage over enzymoanalysis in the precision. But the within-run and between-run CV of enzytnoanalysis were retain within the regulation. There was a good correlation coefficient (r 0. 9767 ; 0. 9685 ;0. 9525)and no significant difference (P> 0. 05). both methodsgive positive interference forpotassium in hemolysis sample For sodium and chloride,there were negative interference in triglyceride byenzymoanalysis and no significant interference by electroanalysis. In general,all the specification of enzymo-analysis fulfil requirements of the routine use although fhe electroanalysis was advantageous.