实验性大鼠脑出血后神经肽Y的变化及抑肽酶的干预作用

Changes of Neuropetie Y After Intracerebral Hemorragic Rats and the Intervention Effects of Aprotinin

目的观察大鼠脑出血后神经肽Y的表达与脑出血后脑含水量变化的关系,以及抑肽酶对其的影响。方法通过采用未抗凝新鲜自体股动脉血注入大鼠尾状核建立脑出血动物模型,用放射免疫法测定脑出血后6h和1、3、5及7天神经肽Y的变化,用干重—湿重法测定脑出血后脑含水量的变化,并与神经肽Y进行相关分析。结果与对照组比较,血肿周围组织中神经肽Y水平在脑出血后1天和3天时显著升高(P<0.05),6天时降至正常。血肿周围组织含水量在脑出血后6h轻度增加,1天时显著增加(P<0.01),3天时达高峰(P<0.01),以后逐渐下降,7天时基本恢复正常水平;抑肽酶组脑含水量及神经肽Y含量均明显减少。结论神经肽Y可能参与实验性脑出血后血肿周围水肿的形成;抑肽酶对二者有抑制作用,抑肽酶可能有助于减轻脑出血后脑水肿的形成。

Aim To investigate dynamic changes of water content and neuropetie Y （NPY） in intracerebral hemorrhagic （ICH） rats and the role that neuropetie Y plays in cerebral edema after hemorrage, and to evaluate the effect of aprotinin in reducing the content of cerebral edema and neuropetie Y. Methods Animal model was made by injecting serf arterial blood into the caudate nucleus of rats. 75 rats were randomly assigned to control group, cerebral hemorrhage group and aprotinin treated group. After the injection, the rats were sacrificed at 6 h, 1 d, 3 d, 5 d, 7 d respectively. Brain water content was determined by wet-dry weight, as the expression of neuropetie Y was detected by radioimmunoassay method over a time course ranging from 6 hours to 7 days. Results Brain water content and neuropetie Y increased after 6 hours of cerebral hemorrhage and reached its climax at the 3 days, then decreased at the 5 to the 7 days; Administration of aprotinin could reduce the content of brain edema and neuropetie Y. Conelusions Aprotinin can reduce neuropetie Y expression, thus can reduce cerebral edema after ICH. Aprotinin antagonist might be available for the therapy of cerebral edema after ICH.