十一酸睾酮对雄性家兔髂动脉内膜损伤后的修复作用

The Effect of Testosterone Undecanoate on Intima Restoration of Iliac Artery After Injury in Male Rabbit

目的观察十一酸睾酮对雄兔髂动脉内膜损伤后的修复作用。方法成熟雄性日本大耳白兔25只,随机分成5组,每组5只:假手术组(不切除睾丸和附睾)、模型组、小剂量组(手术后补充睾酮3mg/kg)、中剂量组(手术后补充睾酮6mg/kg)和大剂量组(手术后补充睾酮12mg/kg),后4组均切除双侧睾丸和附睾。所有动物手术后第14d行右侧髂动脉内皮剥脱术。手术后第7、14和第21d肌肉注射十一酸睾酮,假手术组和模型组肌注生理盐水。于不同时间测定血清总睾酮。第28d处死动物,取损伤血管段,应用扫描电镜和HE染色观察髂动脉内膜的修复情况。结果光镜下观察发现,模型组、小剂量组和中剂量组动脉内膜增生严重,大剂量组内膜增厚程度明显减低,接近假手术组。模型组、小剂量组和中剂量组的髂动脉内膜/中膜面积比值分别是1.25±0.10、0.90±0.10和0.74±0.11,明显高于假手术组(0.22±0.05;P<0.01)。大剂量组的髂动脉内膜/中膜面积比值为0.26±0.07,接近于假手术组(P>0.05)。扫描电镜观察发现,假手术组动物髂动脉内皮光滑,整齐连续,模型组几乎未见到有内皮细胞再生,小剂量组内皮有散在覆盖,中剂量组片状覆盖,大剂量组内皮细胞修复明显,排列趋于正常,偶有缺失。在手术后第28d大剂量组血清总睾酮水平接近假手术组,其它组在不同时间点均低于假手术组。结论生理水平的睾酮具有保护雄性家兔血管内皮,抑制动脉损伤后内膜增生的作用。

Aim To observe the effect of testosterone undecanoate （TU） on intima restoration of iliae artery after injury in male rabbit. Methods Twenty-five male Japanese rabbits were randomized into 5 groups （5 in each） ： control group, castrated group, small dose group （3 mg/kg）, middle dose group （6 mg/kg） and large dose group （12 mg/kg）. All five groups of rabbits were castrated except the control group. At the fourteenth day after castration all animals were performed with endothelium denudation at the right iliac artery. At the seventh, fourteenth and twenty-first day after castration, the last three groups of rabbits were intramuscularly injected with testosterone undecanoate, whereas control group and castrated group were intramuscularly injected with 0.9% saline. Blood sample were collected for determination of total testosterone （TT） at different time. At the twenty-eighth day after castration,all animals were killed by air embohsm. The endothelial structure and morphological changes were observed with scanning electron microscope and HE staining. Results Tile hyperplasia of endometrium was serious in castrated group, small dose group and middle dose group at the sight of light microscope, whereas in large dose group this hyperplasia is as light as that in control group. Tile intima-to-media ratio of ihac artery in castrated group, small dose group and middie dose group （ respectively 1.25 ± 0.10, 0.90 ± 0.10, and 0.74 ± 0.11 ） were obviously larger than those in control group （0. 22 ± 0.05 ） （ P 〈 0.01 ）, whereas no significant difference was found between large dose group （0.26 ± 0.07） and control group （ P 〉 0.05）. Scanning electron microscopy showed that the endothelium of control group was smooth and continuous, and the cells were in good order. Whereas in castrated group almost no endothelial cell was observed. There were a few isolated areas having endothelial cells in small dose and middle dose groups. In large dose group, the arrangement of endothelial cells got close to normal state and have small deletion occasionally. At the twenty-eighth day after castration, serum level of total testosterone （TT） in large dose group was similar to that before castration and in control group. Serum level of TT in other groups was obviously lower than that in control group. Conclusion Testosterone, at physiological level, can protect vascular endothelium and inhibit intima hyperplasia in nlale rabbit.