64例原发性胆囊癌中PTEN和c-erbB-2基因表达

Expression of PTEN and c-erbB-2 in 64 Cases with Primary Gallbladder Carcinoma

[目的]研究PTEN和c-erbB-2基因在原发性胆囊癌(PGC)中的表达及其意义。[方法]应用原位杂交(DNA-RNA)技术与免疫组化SP法分别检测64例PGC中PTEN mRNA、PTEN蛋白和c-erbB-2蛋白的表达。以22例胆囊腺瘤及10例慢性胆囊炎作为对照。[结果]PTEN mRNA和蛋白在PGC组织中的阳性表达率(56.3%,59.4%)均明显低于胆囊腺瘤组织(81.8%,90.8%)和慢性胆囊炎组织(100%,100%)(P<0.05)。c-erbB-2蛋白在PGC组织中的阳性表达率(46.8%)均明显高于胆囊腺瘤组织(9.1%)和慢性胆囊炎组织(0)(P<0.05)。[结论]PTEN低表达和c-erbB-2高表达共同参与了PGC的发生发展,其蛋白可作为判断PGC预后的指标。

[Purpose ] To study the expression of PTEN and c-erbB-2 in primary gallbladder carcinoma（PGC） and its significance. [Methods ] The expression of PTEN mRNA, PTEN protein and c-erbB- 2 protein were detected by in situ hybridization（DNA-RNA） and inmmnochemistry（SP method） in 64 cases with PGC, 22 cases with gallbladder adenoma and 10 cases with chronic cholecystitis as controis. [Results ] The positive expression rates of PTEN mRNA and PTEN protein in PGC （56.3%, 59.4%） iwere significantly lower than those in gallbladder adenoma （81.8%,90.8%） and chronic cholecystitis（100%,100%）（P〈0.05）. The positive expression rate of c-erbB-2 protein in PGC（46.8%） was significantly higher than that in gallbladder adenoma （9.1%） and chronic cholecystitis （0）（P〈0.05）. [Conclusion ] Low expression of PTEN and high expression of c-erbB-2 both play an important role in oncogenesis of PGC. They may be uses as markers for prognosis evaluation.