豹皮樟总黄酮对免疫低下小鼠免疫功能的影响

Immunomodulatory property of total flavonoids from Litsea coreana leveille on immunosuppressive mice

目的研究豹皮樟总黄酮(litsea coteana total flavone,LCTF)对环磷酰胺(cyclophosphamide,Cy)诱导的免疫低下小鼠免疫功能的影响。方法以Cy50mg·kg-1ip给药,每天1次,连续2d,诱导小鼠免疫低下模型,采用碳廓清试验、溶血素生成试验、二硝基氟苯(DNFB)诱导的迟发性超敏反应,观察LCTF对免疫低下小鼠非特异性免疫、体液免疫和细胞免疫的影响。结果碳廓清试验,LCTF(200、400mg·kg-1)组可提高Cy致免疫低下小鼠的吞噬指数α值和廓清指数K值,提高免疫低下小鼠巨噬细胞的吞噬功能;溶血素试验,LCTF(100、200mg·kg-1)组能提高免疫低下小鼠血清IgM和IgG的生成,LCTF(100、200、400mg·kg-1)能增加免疫低下小鼠脾细胞溶血素的产生;LCTF(200、400mg·kg-1)组可明显促进免疫低下小鼠DTH反应,LCTF(100、200、400mg·kg-1)能提升CD4+、CD8+细胞数以及CD4+/CD8+比值,并能提高脾淋巴细胞生产IL-2含量。结论以上结果表明,LCTF通过广泛刺激小鼠的特异性及非特异性免疫应答,对免疫低下小鼠的免疫功能有良好的增强作用。

Aim To study the immunomodulatory activity of total flavonoids of Litsea Coreana Leveille （LCTF） on cyclophosphamide （CY） -induced immunosuppressive mice. Methods CY （50mg·kg^-1 ） was administered by intraperitoneal（ip） injection for 2 consecutive days to induce immunosuppressive model. Carbon clearance, quantitative hemolysis and DNFB-induced delayed-type hypersensitivity （DTH） were applied to assay effects of LCTF on nonspecific immunity, humoral immunity and cellular immunity. Results In carbon clearance test, the clearance index （K） and values of phagocytic index （α） were elevated by LCTF （ 200 and 400 mg·kg^-1） , indicating the phagocytosis of macrophages was enhanced in immunosuppressive mice. In quantitative hemolysis, productions of IgM and IgG in serum and hemolysin in splenocytes were enhanced in immunosuppressive mice by LCTF （100 and 200 mg·kg^-1 ）. LCTF （200 and 400 mg·kg^-1） obviously increased DTH reactivity in immuno- suppressive mice. LCTF not only increased percentages of T cells expressing CD4^＋ and CD8^＋, but also enhanced the ratio of the two subset of T lymphocyte, and LCTF （200 and 400mg·kg^-1） could also improve IL-2 production of spleen lymphocytes. Conclusion LCTF showed significant immunomodulatory property on immunosuppressive mice specific immunity. through specific and non-specific immunity.