期刊文献+

改进的DNA指纹技术在玉米杂交种纯度鉴定上的应用研究 被引量:2

A Rapid DNA Extraction Procedure for Maize Purity Test
下载PDF
导出
摘要 本试验以幼苗为对照,利用CTAB法建立了一套简单、快速的玉米干种子DNA提取程序,该程序提取的DNA分子量大小与幼苗法相当,能够利用SSR引物进行扩增,可应用于玉米杂交种纯度检测。 Two sets of DNA samples were extracted from dry maize seeds with two different treatments using CTAB method. Compared with the DNA extracted from seedlings, they had the some molecular weight and were suitable for PCR amplification. So it was a rapid and simple DNA extraction method for maize purity test.
出处 《山东农业科学》 2007年第5期42-44,共3页 Shandong Agricultural Sciences
关键词 玉米 杂交种 DNA提取 纯度 SSR标记 Maize Hybrid DNA extraction Purity SSR marker
  • 相关文献

参考文献11

二级参考文献37

  • 1余四斌,徐才国.用RAPD技术鉴定水稻种子纯度初探[J].种子,1996,15(5):56-57. 被引量:45
  • 2王玉民,庄炳昌,邓祟辉.玉米半粒种子DNA提取及RAPD分析[J].玉米科学,1996,4(3):27-28. 被引量:16
  • 3傅骏骅,李连城,刘新芝,彭泽斌,黄长玲.玉米RAPD程序优化研究及其初步探讨[J].作物学报,1997,23(1):56-61. 被引量:49
  • 4郭景伦,赵久然,尉德铭,郭强,王斌,张超良,金德敏,李荣旗,张开春.玉米单粒种子DNA提取新方法[J].北京农业科学,1997,15(2):1-2. 被引量:87
  • 5Smith J S C, et al. An evaluation of the utility of SSR loci as molecular markers in maize ( Zea mays L. ): comparisons with date from RFLPs and pedigress[C]. UPOV working group on biochemical and molecular technique and DNA profiling in particular fourth
  • 6Smith J S C and Register J C.G enetic purity and testing technologies for seed quality: a company perspective[J].Seed Science Research, 1998 ,8: 285 - 293.
  • 7Powell W, Marchray G C, Provan. Polymorphism revealed by simple sequence repeats [J]. Trends in Plant Science, 1996,1:215 - 222.
  • 8Senior M I, Heun M. Mapping maize Microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT primer[J]. Genome, 1993,36: 884 - 889.
  • 9Weising,et al. DNA Fingerpringting in Plants and Fungi[M] .CRC Press Inc., 1995,51 - 55.
  • 10Matsuoka Y, et al. Microsatellites in Zea- variability, patterns of mutations, and use for evolutionary studies [ J ]. Theor. Appl. G enet,2002,104:436 - 450.

共引文献245

同被引文献12

引证文献2

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部