黑皮素-1受体在增生性瘢痕组织中的表达与分布

The study of expression and distribution of melanocortin-1 receptor in hypertrophic scar

目的:研究黑皮素-1受体(mel anocort in-1 recept or,MC-1R)在增生性瘢痕组织中的表达和分布特点,探讨它与增生性瘢痕形成的关系。方法:利用兔抗人MC-1R抗体进行免疫组织化学染色,并测定8例增生性瘢痕,8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值,分别检测MC-1R在上述不同组织中表达的差异。结果:在正常皮肤、表浅瘢痕和增生性瘢痕中,MC-1R免疫阳性产物呈棕黄色颗粒状,主要分布于表皮基底层细胞胞浆中。在增生性瘢痕组织中,MC-1R灰度值(159.2±5.5)明显高于正常皮肤(134.7±5.9)和表浅性瘢痕(135.9±9.3),差异显著(P<0.01)。而后两者无明显差异(P>0.05)。结论:增生性瘢痕的形成可能与MC-1R在增生性瘢痕组织中表达明显减少,由其介导的α-黑色素细胞刺激素(α-melanocyte stimul ating hormone,α-MSH)对胶原蛋白的代谢和细胞外基质沉积的调节作用减弱有关。

Objective To observe the expression and the distribution of melanocortin-1 receptor （MC-1R） in hypertrophic scar and then identifying the relationship between MC-1R and formation of hypertrophic scar. Methods Bioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample. Results In normal skin, superficial scar and hypertrophic scar, MC-1R immune positive material was yellow-brown granule, mainly distributed in plasma in basal lamina of epidermal, semi quantitative diction showed that the mean of MC-1R grey scale in hypertrophic scar（159.2±5.5） was significantly higher than that of normal skin （134.7±5.9）and superficial scar （135.9±9.3）, respectively （P〈0.01）. But, there was no significant deference between normal skin and superficial scar.Conclusion Our result suggested that the formation of hypertrophic scar was possibly relative to the decrease in expression of MC-1R in hypertrophic scar and then the regulation of α-MSH by MC-1R in the sediment of extracellular matrix.