猪胆汁性肝硬化模型的造模及评价与分析

Evaluation and Analysis Model of Biliary Cirrhosis of Porcine

目的探讨手术结扎总胆管制备胆汁性肝硬化模型的成功率,寻求评估肝储备功能、肝硬化程度的方法。方法选择普通猪10头,剖腹、游离结扎胆总管,定期取材采样进行相关检测、测量门静脉压。结果4头猪分别于第3、45、周死亡,肝硬化模型死亡率为40%,肝硬化形成率100%,用药早期,ALT、AST呈逐渐上升趋势,后期ALT、AST趋于平稳,并有下降的趋势,总胆红素持续上升;白蛋白持续下降。结论肝硬化模型成功率较高,造模时间短,但死亡率较高,病理活检仍是目前确诊肝纤维化的金标准,ALT、AST变化对于评价肝硬化的发展无意义;白蛋白、总胆红素水平在肝硬化形成不同阶段具有统计学差异,对于评价肝硬化进程具有一定的价值,但灵敏性较低,积极寻求对肝硬化进展程度评估价值较高指标是今后努力的目标。

Objective To discuss achievement ratio and mortality rate of preparation of porcine model of biliary cirrhosis, and seek methods to evaluate hepatic function reserve. Methods Choosing ten common pigs as experimental animal, cutting the belly open, dissociating and ligating common bile duct, during the course of the experimental procedure, we collect samples and measure portal venous pressure regularly. Results The rate of success is 100 percent and the rate of mortality is 40 per cent. ALT and AST go up step by step in the early stages, then go steady. TBIL increases continuously, on the contrary, albumin decreases continuously. Conclusion Rate of success of model of hepatic cirrhosis is more higher and time which is needed is shorter, but the rate of mortality is more higher. Pathology biopsy is still a gold standard of diagnosing fibrosis at present. The changes of ALT and AST have no significance for evaluating the development of hepatic cirrhosis. Albumin and bilirubin have obviously difference in before- and after-liver cirrhosis, which is useful to evaluate liver cirrhosis, but sensibility is poor, we should take active in seeking more valuable indexes.