硒酸精氨酸对小鼠酒精性肝损伤的保护作用

Protection of Selenoarginine(SeArg) against Alcohol-induced Liver Injury in Mice

目的探讨硒酸精氨酸对小鼠酒精性肝损伤的保护作用。方法用连续灌服酒精的方法建立小鼠酒精性肝损伤模型。将60只雄性小鼠随机分为四组:对照组,模型组,硒酸精氨酸处理组和二氧化硒处理组。连续灌胃8周后,测定小鼠血清中天冬氨酸氨基转移酶(AST)、丙氨酸转移酶(ALT),肝匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽(GSH)的含量。对肝脏切片做HE染色和Masson染色,在光镜下观察肝脏病理学变化。结果硒酸精氨酸能显著降低酒精性肝损伤引起的AST的升高(P<0.01),有效保持了肝脏中GPx和SOD的活性(P<0.01)。病理学观察结果与酶学变化相一致,硒酸精氨酸对肝脏的保护作用明显优于二氧化硒。结论硒酸精氨酸对小鼠酒精性肝损伤具有显著的保护作用,效果也优于二氧化硒。

Objective To study the protective effect of selenoarginine against alcohol-induced liver injury in mice. Methods Alcohol-induced liver injury mouse model was set up by executive intragastric administration. 60 male mice were randomly divided into four groups： control group, model group, selenoarginine group and SeO2 group. The level of aspartate tmnsaminases （AST） and alanine transaminases （ALT） in serum, and superoxide dismutase （SOD）, glutathione peroxidase （GPx）, glutathione （GSH） of liver homogenate were measured. Liver sections were stained with Hematoxylin ＆ Eosin or Masson, and observed under the microscope. Results Selenoarginine significantly reduced the elevation of AST （ P 〈 0.01 ） caused by alcohol, and maintained the activity of SOD and GPx （P 〈 0.01 ）. Histopathological observations were consistent with biochemical changes, suggesting marked protective effect of selenoarginine compared with SeO2. Conclusion Selenoarginine had an obviously protective effect against alcohol-induced liver injury, and performed better than SeO2.