四川道地药材泽泻指纹图谱的建立及其质量研究

Establishment of chromatographic fingerprint and quality assessment on Sichuan native medicinal plant Alisma plantago-aquatica

目的:建立泽泻药材的指纹图谱,研究四川不同产区泽泻药材的质量,为科学评价及有效控制其质量提供参考。方法:采用梯度洗脱法进行色谱分离,使用"中药指纹图谱相似度计算软件"进行数据处理。结果:17批样品得到的色谱指纹图谱有26个共有峰,16和22号峰分别被鉴定为24-乙酰泽泻醇A和23-乙酰泽泻醇B。结论:泽泻药材的指纹图谱特征性及专属性强,可结合含量测定用于全面控制其质量,确保每批产品的均一性。

Objective： To establish chromatographic fingerprint of Sichuan native medicinal plant Alisma plantago-aquatica by RP-HPLC for the quality control. Method： The gradient elution mode was applied in chromatographic separation, and data were analyzed by ＂Computer Aided Similarity Evaluation＂ software to compare the quality of A. plantago-aquatica samples from different habi- tats. Result： The HPLC chromatographic fingerprinting of A. plantago-aquatica with 26 characteristic peaks was established from 17 lots of A. plantago-aquatica samples, peak 16 and 22 were identified as 24-aeetyl alisol A and 23-acetyl alisol B, respectively. Conclusion： The chromatographic fingerprinting of A. plantago-aquatica with high specificity can be used to control its quality and assure lot to lot consistency. The RP-HPLC fingerprint method is repeatable, feasible in analysis of A. plantago-aquatica.