肉苁蓉种子发芽条件研究

Study on conditions of seed germination of Cistanche

目的:明确氟啶酮预处理浓度、时间、发芽温度和盐分对3种肉苁蓉种子发芽的影响。方法:用培养皿法培养种子,统计发芽率。结果:0.1mg·L^(-1)氟啶酮预处理的管花肉苁蓉、荒漠肉苁蓉和盐生肉苁蓉种子发芽率最高,预处理时间为24～29h。适宜温度为20～30℃,低于5℃或高于35℃,种子不能发芽。盐生肉苁蓉种子耐盐性强,NaCl浓度临界值为0.04mol·L^(-1),管花肉苁蓉和荒漠肉苁蓉种子耐盐性较弱,NaCl浓度临界值为0.02 mol·L^(-1)。结论:3种肉苁蓉种子发芽率测定方法和条件是:用0.1mg·L^(-1)氟啶酮预处理24h后,温度为20～30℃,盐分低于0.02mol·L^(-1)。

Objective： To study the effect of fluridone concentration, stimulating period, temperature and salt on the seed germination of three species of Cistanche. Method： The seeds were cultured in Petri dish, and the germination percentage was counted. Result： The highest germination percentage was observed in Cistanche tubulosa, C. deserticola, C. sala seeds pre-treated by 0. 1 mg · L^-1 fluridone for 24-29 h. The optimal temperature for the seeds germination of three species of Cistanche was at 20-30 ℃, and the seeds did not germinate at sub-or supraoptimal temperatures （5 and 35 ℃ ）. The salt tolerance of C. sala seeds was strong, and the critical value of NaCl concentration was 0. 04 mol · L^-1. By contrast, C. tubulosa and C. deserticola seeds were more sensitive to the salt stress, the critical value of NaCl concentration was 0. 02 mol · L^-1. Conclution： The optimal germination condition and the method of testing germination percentage of three species of Cistanche seeds ate as follow： the seeds are pre-treated by 0. 1 mg· L^-1 fluridone for 24 h and then cultured at 20-30 ℃ in salt solution which concentration is lower than 0. 02 mol· L^-1.