摘要
目的:明确氟啶酮预处理浓度、时间、发芽温度和盐分对3种肉苁蓉种子发芽的影响。方法:用培养皿法培养种子,统计发芽率。结果:0.1mg·L^(-1)氟啶酮预处理的管花肉苁蓉、荒漠肉苁蓉和盐生肉苁蓉种子发芽率最高,预处理时间为24~29h。适宜温度为20~30℃,低于5℃或高于35℃,种子不能发芽。盐生肉苁蓉种子耐盐性强,NaCl浓度临界值为0.04mol·L^(-1),管花肉苁蓉和荒漠肉苁蓉种子耐盐性较弱,NaCl浓度临界值为0.02 mol·L^(-1)。结论:3种肉苁蓉种子发芽率测定方法和条件是:用0.1mg·L^(-1)氟啶酮预处理24h后,温度为20~30℃,盐分低于0.02mol·L^(-1)。
Objective: To study the effect of fluridone concentration, stimulating period, temperature and salt on the seed germination of three species of Cistanche. Method: The seeds were cultured in Petri dish, and the germination percentage was counted. Result: The highest germination percentage was observed in Cistanche tubulosa, C. deserticola, C. sala seeds pre-treated by 0. 1 mg · L^-1 fluridone for 24-29 h. The optimal temperature for the seeds germination of three species of Cistanche was at 20-30 ℃, and the seeds did not germinate at sub-or supraoptimal temperatures (5 and 35 ℃ ). The salt tolerance of C. sala seeds was strong, and the critical value of NaCl concentration was 0. 04 mol · L^-1. By contrast, C. tubulosa and C. deserticola seeds were more sensitive to the salt stress, the critical value of NaCl concentration was 0. 02 mol · L^-1. Conclution: The optimal germination condition and the method of testing germination percentage of three species of Cistanche seeds ate as follow: the seeds are pre-treated by 0. 1 mg· L^-1 fluridone for 24 h and then cultured at 20-30 ℃ in salt solution which concentration is lower than 0. 02 mol· L^-1.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第18期1848-1850,共3页
China Journal of Chinese Materia Medica
基金
国家科技攻关项目(2001BA701A24-10)
河北省科技攻关项目(03276408D-4)
新疆维吾尔自治区科技攻关项目(200433117)
关键词
肉苁蓉
发芽率
种子
条件
氟啶酮
Cistanche
germination percentage
seed
condition
fluridone