重点保护药用植物金毛狗配子体发育过程的研究

Observation on gametophyte development of Cibotium barometz

目的:通过对金毛狗孢子萌发的研究,了解其生长发育规律,为金毛狗的室内培养与进一步研究提供理论和实践依据。方法:用土壤和Knop's培养基在室内培养金毛狗孢子,并在显微镜下观察记录孢子萌发及配子体发育全过程。结果:孢子播种1～2周左右萌发,萌发类型为书带蕨型;播种25d左右发育为片状体,毛状体出现在片状体形成之后,40d左右形成原叶体,成熟原叶体呈心脏形;精子器在接种后60d左右开始产生,颈卵器在精子器发生后10d左右形成。结论:室内土壤培养金毛狗孢子效果好,方法可行,是快速发展金毛狗的一条捷径。

Objective： To observe the spores germinating process of Cibotium barometz,and understand the growth principle provided for experience for indoor culturing and further research. Method ：The spores of C. barometz were cultured beth in inorganic medium and in the soil from original habitat, and the whole process of spores germination and the development of gametophytic were observed under microscope. Result：The spores germinated about 1-2 weeks after being sowed, and the type of germination belonged to Vittaria-type. The prothallial plates formed in 25 days after being sowed, while hairs developed after the formation of the prothallial plate. The gametophyte formed about 40 days after being sowed. But the type of mature prothalli was cordate. The antheridia formed in 60 days after inoculation, while the archegonia developed in 10 days after the formation of antheridia. Conclusion：Soil based indoor culturing of C. barometz spores is practical and can be used for cultivation of C. barometz.