RP-HPLC测定白土茯苓中甲基氧化偶氮甲醇樱草糖苷的含量

Determination of macrozamin in Rhizoma Heterosmilacis Japonicae by RP-HPLC

目的:建立白土茯苓中甲基氧化偶氮甲醇樱草糖苷的RP-HPLC定量分析方法。方法:采用Century C_(18)AQ色谱柱(4.6mm×250mm,5μm),以甲醇.水(4:96)为流动相,流速为1.0mL·min^(-1),检测波长为215nm,柱温35℃。结果:甲基氧化偶氮甲醇樱草糖苷在19.12～382.4μg·mL^(-1)线性关系良好(r=0.9998),方法回收率为99.5%,RSD为2.1%(n=9)。结论:该方法可用于白土茯苓的质量控制。

Objective： To develop a RP-HPLC method for the determination of the content of macrozamin in Rhizoma Heterosmilacis Japonicae. Method： A Century C18 AQ column （4. 6 mm×250 mm, 5μm） was used with the mobile phase consisted of water （4： 96）. The flow rate was 1.0 mL·min^-1. The detection wavelength was set at 215 nm, and the column temperature was 35℃. Result： The calibration curve was hnear （r =0. 999 8） in the range of 19. 12-382.4 μg · mL^-1 for macrozamin, the average recovery of the method was 99. 5 %, with RSD 2. 1% （ n = 9）. Conclusion： This method can be used for the quality study of Rhizoma Heterosmilacis Japonicae.