绵萆薢对去卵巢大鼠骨代谢改变的干预作用

Effects of Dioscorea septemloba on bone metabolism in ovariectomized rats

目的:探讨绵萆薢对去卵巢大鼠骨代谢的影响。方法:3月龄雌性未孕大鼠30只,随机分为假手术组,去卵巢组,己烯雌酚组(4.3×10^(-4)g·kg^(-1)·d^(-1)),绵萆薢高、中、低剂量组(4,2,1g·kg^(-1)·d^(-1)),每组均为5只大鼠。连续给药12周后,取血、尿用于血清雌二醇(E_2)、钙(Ca)、磷(P)、骨钙素(BGP)、碱性磷酸酶(ALP),尿钙/肌酐(Ca/Cr)、磷/肌酐(P/Cr)、脱氧吡啶啉/肌酐(DPD/Cr)的测定;分离子宫并称重,测量股骨骨密度和生物力学指标。结果:绵萆薢各剂量组子宫指数均明显高于模型组(P<0.01)。去势大鼠血清ALP,BGP,尿DPD/Cr,Ca/ Cr含量均比假手术组明显升高(P<0.05),而绵萆薢各剂量组血清BGP,ALP,尿DPD/Cr,Ca/Cr含量均低于模型组。各组血Ca,P,尿P/Cr均无显著性改变。去势组大鼠骨密度为(0.032±0.007)g·cm^(-2),与假手术组比较显著降低(P<0.01),绵萆薢各剂量组骨密度与模型组大鼠比较均明显升高(P<0.05,P<0.01)。去势大鼠最大载荷、挠度、最大应变分别为(125.78±15.48)N,(1.87±0.22)mm,(9.34±1.10)%,均显著低于假手术组(P<0.05,P<0.01);各剂量绵萆薢可以不同程度地提高其最大载荷、最大应力。结论:绵萆薢可降低骨转换,使去卵巢大鼠骨丢失得到改善。

Objective： To investigate the effects of the decoction of Rhizoma Dioscorea septemlobae （RD） on the bone metabolism in ovariectomized rats. Method： Thirty female, 3-month-old Wistar rats without pregnancy and deliver were randomly divided into 6 groups ： sham （ sham-operation）, ovariectomy （ OVX）, OVX ＋ diethylstilbestrol, OVX ＋ high dose RD （4 g · kg^-1·d^-1 ）, OVX ＋ middle dose RD （2 g · kg^-1·d^-1 ） and OVX ＋ low dose RD （ 1 g · kg^-1·d^-1 ） （ n = 5 in every group）. After 12-week period of continuous treatment, the urinary samples and blood samples were collected for the determination of serum estrodiol （ E2 ）, calcium （Ca）, phosphorus （ P）, bone glaprotein （ BGP）, alkaline phosphatase （ ALP）, urinary calcium/creatinine （ Ca/Cr）, phosphorus/ creatinine （P/Cr） and deoxypyridioline/creatinine （DPD/Cr）. The uteri were removed and weighed. The bone mineral density （BMD） and the biomechanical parameters of the femur of the rats in every group were determined, respectively. Result： The coefficient of uteri in every dose group of OVX ＋ RD was significantly higher than that in the OVX group （P 〈0. 01 ）. The concentration of serum ALP, BGP and urinary DPD/Cr, Ca/Cr in the OVX group was significantly higher than that in the sham group （P 〈0. 05）, respectively, However, that in the every dose of OVX ＋ RD was lower than that in the OVX group, respectively. There was no significan difference in the concentration of serum Ca, P and urinary P/Cr in every group, respectively. The bone mineral density （BMD） in the OVX group was （0. 032±0. 007） g · cm^-2 and was significantly lower than that in the sham group （P 〈0. 01 ）. However, the value in the group of every dose OVX ＋ RD was significantly higher than that in the OVX group （ P 〈 0. 05, P 〈 0. 01 ）, respectively. The maximum loading, deflection and the maximum strain of the femur in the OVX group were （ 125. 78 ± 15.48 ） N, （ 1.87 ± 0. 22） mm, （9. 34 ± 1.10）% and were significantly lower than those in the sham group （P 〈0. 05, P 〈0. 01 ）, respectively. The maximum loading and maximum stress were increased in different extent in the every dose group of OVX ＋ RD, respectively. Conclusion： The decoction of RD can inhibit bone absorption, decline bone turnover and improve the loss of bone in ovariectomized rats.