摘要
目的探讨不同浓度白介素-8（IL-8）在自体脂肪颗粒移植成活中的作用。方法在自体脂肪颗粒移植的实验动物模型中，应用不同浓度的IL-8（A组100ng／ml、B组200ng／ml、C组400ng／ml、D组800ng／ml）移植40d后，分别采集移植的脂肪组织，对不同实验组和对照组移植物质量变化进行比较，并行HE染色观察移植组织病理学变化及免疫组织化学染色对组织中血管密度行定量研究，观察血管生长情况。结果各实验组移植物质量改变与对照组没有显著差异。组织病理学分析显示，后3组移植体中包囊和空泡的形成明显较对照组减少，微血管显影清晰，并且这3组微血管定量亦较对照组高。结论IL-8可以有效改善自体脂肪颗粒移植物的血流灌注，减少包囊的生成，提高移植脂肪质量，且这种作用可能与IL-8的浓度有关。
Objective To investigate the effects of interleukin-8 on the survival of autologous pearl fat transplantation with different concentrations. Methods Interleukin-8 of different concentrations （ group A 100 ng/ml, group 15 200 ng/ml, group C 400 ng/ml, and group D 800 ng/ml） was used in pearl auto-fat transplantation in experimental groups. And 0.9 % NS was used in the control group. After 40 days graft weights were measured and histologic evaluation was performed. In addition, microvessel densities were quantitatively detected in various groups. The growth course of vessels was observed. Results Histologic analysis demonstrated significantly less cyst and vacuoles formation in the experimen- tal groups B, C and D than that in control group. Microvessels were clearly observed. The microvessels densities in these groups were higher than that in control group. No significant differences were found in the groups with regard to graft weight. Conclusions Interleukin-8 can effectually accelerate the growth of blood vessels in autologous pearl fat graft and obtain less cystic formation, indicating improved quality of the injected fat. This effect may be relative to the concentration of IL-8.
出处
《中华医学美学美容杂志》
2007年第4期200-203,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
