山药多糖的分离纯化及组成研究

Study on Constitutes,Separation and Purification Technology of Yam Polysoccharides

本实验研究了山药多糖的提取工艺,得出最佳工艺条件为料液比1:25,100℃下浸提2.5h,此时山药多糖的提取率最大。在进一步的利用DEAE52,Sephadex G-100分离纯化中,我们发现了三种多糖,包括中性多糖和两种酸性多糖。利用GC-MS分析结果显示,三种多糖的单糖组成分别为:中性糖由葡萄糖和甘露糖组成,酸性多糖1是由葡萄糖、半乳糖、甘露糖组成。酸性糖2是由阿拉伯糖、木糖、阿卓糖、葡萄糖、甘露糖组成。

This paper studied the separation technics of the yam polysaccharides. The results showed the optimum conditions to separate the polysaccharide are： S/W 1：25, tempetrature 100 ℃ and time 2.5 h. During the next purification step, three kinds of polysaccharides are found, including one neutral polysaccharide and two acid polysaccharides. The GC-MS results showed that the neutral polysaccharide includes glucose and mannose, but acid polysaccharide 1 includes glucose, mannose and galactose, acid polysaccharide 2 includes glucose, mannose arabinose, xylose and altrose.