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HPLC-TLC/HPLC-MS法分离、鉴定与测定二亚油酰磷脂酰胆碱 被引量：2

Separation,Identification and Determination of Dilinoleoyl-phosphatidylcholine by HPLC-TLC/HPLC-MS
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摘要 应用HPLC/HPLC-MS研究了二亚油酰磷脂酰胆碱的测定方法,磷脂样品首先经过HPLC分离得到磷脂酰胆碱,该磷脂酰胆碱再进一步通过不同的HPLC系统分离二亚油酰磷脂酰胆碱,并通过标准样品和质谱进行二亚油酰磷脂酰胆碱的定性。定量方法通过标准样品外标法定量,由方法的回收率和精密度实验可知,该方法用来测定一般磷脂样品和磷脂酰胆碱样品中的二亚油酰磷脂酰胆碱含量,方法准确、可靠、简便。 The analytical method of dilinoleoyl-phosphatidylcholine was studied in this paper. The phosphatidylcholine was separated out from phospholipid by HPLC. The dilinoleoyl-phosphatidylcholine was futher separated out from the phosphati- dylcholine by another HPLC system. Qualitative analysis of dilinoleoyl-phosphatidylcholine was completed with the aid of standard sample and MS （mass spectrum）. Finally quantitative analysis of dilinoleoyl-phosphatidylcholine was completed with the aid of standard sample. It was known by tests of method reclamation and precision that the method is accurate, reliable, and handy in the analysis of dilinoleoyl-phosphatidylcholine from phospholipid or phosphatidyl- choline.
作者 曹栋
机构地区 江南大学食品学院
出处 《食品科学》 EI CAS CSCD 北大核心 2007年第9期442-445,共4页 Food Science
关键词 二亚油酰磷脂酰胆碱 磷脂酰胆碱 高效液相色谱 dilinoleoyl-phosphatidylcholine phosphatidylcholine HPLC
分类号 TS207.5 [轻工技术与工程—食品科学]
  • 相关文献

参考文献9

  • 1曹栋,裘爱泳,王兴国.大豆磷脂酰胆碱分子类型的研究[J].中国油脂,2003,28(12):49-52. 被引量：13
  • 2PARKS S. Phosphatidylcholine fluidity and sllalctttre affect lecithin: holesterol acyltramferase activity[J]. Journal of Lipid Research, 2000, 41: 546-553.
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  • 9NAVDER. PPC decreases alcoholic hyperlipemia without affecting the alcohol-induced rise of HDL-cheolesterol[J]. Life Sci, 1997, 61: 1907- 1914.

二级参考文献1

共引文献12

同被引文献15

  • 1刘梅,陆伦根,曾民德.多烯磷脂酰胆碱对肝细胞保护机制的研究进展[J].肝脏,2006,11(1):43-45. 被引量：98
  • 2Navder K D. Oxidation of LDL in baboons is increased by alcohol and attenuated by polyenylphosphatidylcholine [ J ]. Journal of Lipid Research, 1999, 40: 983-986.
  • 3Reaven. Effects of oleate- rich and linoleate - rich diets on the susceptibility of LDL to oxidative modification in mildly hypercholesterolemic subjects[J]. Clin Invest, 1993,91 (2) : 668-676.
  • 4Yip Shiu - Hang Henry, Ashraf - Khorassani. Analytical scale supercritical fluid fractionation and identification of single polar lipids from deoiled soybean lecithin[ J]. Journal of Separation Science, 2008, 31 (8) : 1290 -1298.
  • 5Began G, Manohar B. Udaya K Sankar. Enrichment of phosphatidylcholine alcohol fractionation of carbon dioxide de - oiled lecithin from soybean (Glycine max) [ J]. Z Lebensm Unters Forsch A, 1997, 205: 360 -363.
  • 6李桂华,张根旺,高风海.磷脂酰胆碱的制备方法:授权公告日,2007年3月23日.中国专利号,编号[P].公告日期200410060457.8.
  • 7曹栋.二亚油酰磷脂酰胆碱分析[J].分析试验室,2007,26(3):11-14. 被引量：2
  • 8王浩渊,赵擎宇.脓毒症肝损害机制和多烯磷脂酰胆碱的抗炎护肝作用研究进展[J].肝脏,2007,12(4):313-315. 被引量：7
  • 9Parks JS, Huggins KW, Gebre AK, et al. Phosphatidylcholine fluidity and structure affect lecithin: cholesterol acyltransferase activity[J]. J Lipid Res, 2000, 41(4): 546-553.
  • 10Zierenberg O, Grundy SM. Intestinal absorption of polyenephosphatidylcholine in man[J]. J Lipid Res, 1982, 23(8): 1136-1142.

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食品科学
2007年 第9期

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