壳聚糖与知母配伍对糖尿病小鼠晚期糖化终末产物的影响

Effects of Chitosan and It's Rhizoma anemarrhenae Mixture on Advanced Glycosylation End Products (AGEs) of Diabetic Mice

目的:探讨壳聚糖及其与知母配伍对糖尿病小鼠晚期糖化终末产物的抑制和降糖作用。方法:四氧嘧啶腹腔注射建立糖尿病小鼠模型,将小鼠分为正常组(normal)、模型组(model)、壳聚糖组、壳聚糖+知母组(高、低),连续灌胃28d,取血清测定生化指标。结果:与正常组相比,模型组血糖、果糖胺、游离脂肪酸、晚期糖化终末产物(AGEs)、MDA明显升高,pH、SOD明显降低。与模型组相比,壳聚糖组MDA、血糖和AGEs明显降低,SOD明显升高;壳聚糖+知母高剂量组MDA显著降低,血糖和AGEs明显降低,SOD明显升高;各组pH值明显升高。结论:壳聚糖及其与知母配伍对糖尿病小鼠有保护作用。

Objective： The purpose was to evaluate the efficacy of chitosan and it＇s Rhizoma anemarrhenae mixture on regulating blood glucose and advanced glycosylation end products（AGEs） in diabetic mice bodies. Method： Diabetes mellitus model was established by intraeritoneal injecting of alloxan, and the mice were randomly divided into normal group, model group, chitosan group, chitosan ＋ Rhizoma anemarrhenae group（high and low）. The mice were treated with drugs intragastrically（ig） for 28 days, the following parameters were evaluated： serum glucose, fructosamine, free fatty acid（FFA）, serum advanced glycosylation end products（AGEs）, serum SOD and MDA. Results： Compared with normal group, the blood glucose, AGEs ,fructosamine, FFA, MDA in model group were increased, while pH and SOD was decreased in the model group. Compared with model group, MDA, blood glucose, AGEs and FFA were reduced, while SOD were increased in chitosan or the higher mixture group, the PH were increased in the treatment groups, and no significant differences were tested of fructosamine in the treatment groups. Conclusion： Chitosan, chitosan ＋ Rhizoma anemarrhenae could protect the diabetic mice by reducing blood glucose and blood AGEs.