LOX-1 3′-UTR+1073多态性与冠心病的相关性

Association of polymorphism of LOX-1 3′-UTR+1073 with coronary heart disease in Chinese population

目的探讨血凝素样氧化低密度脂蛋白受体(LOX)-1基因中3′-UTR+1073位点(C/T)基因型多态性与冠心病的遗传易感性的关系。方法采用PCR和限制性片段长度多态性(RFLP)分析方法对LOX-1 3′-UTR+1073位点C/T基因型多态性进行检测,观察其在101例冠心病患者(其中61例心肌梗死患者)和54例对照中的频率分布。并与冠心病危险因素和临床情况相结合进行综合分析。结果①LOX-1 3′-UTR+1073基因CC、CT与TT基因型及C、T等位基因在冠心病组和对照组中分布频率无显著差异;在心肌梗死组和对照组中分布频率无显著差异;②在具有相同危险因素(高血压、糖尿病、吸烟)的人群中,3′-UTR+1073基因CC、CT与TT基因型在患冠心病和不患冠心病者分布频率无显著意义。结论LOX-1 3′-UTR+1073基因的基因型多态性与冠心病可能无关,不能作为冠心病的遗传易感标志。

AIM To explore whether at the site of 3′-UTR ＋ 1073 in the gene of LOX-1, genotype polymorphism is the hereditary susceptible embodiment of coronary heart disease. METHODS Adopting PCR and RFLP method, we detected genotype polymorphism at the site of 3′-UTR ＋ 1073 and observed the frequency distribution in 101 cases of coronary heart disease patients （ including 61 cases MI patients） and 54 control cases. The results were analyzed together with risk factors of coronary heart disease and clinical manifestations. RESULTS No obvious distribution frequency difference of gene LOX-1 3′- UTR ＋ 1073 genotype CC, CT and TT, and allele C and allele was observed between CHD and control groups , and between MI and control. No obvious different distribution frequency of geneotype CC, CT and TT of LOX-1 3′-UTR ＋ 1073 gene was observed among the cases with the same risk factors （ hypertension, diabetes and smoking） and cases with CHDs and without-CHDs. CONCLUSION It is likely that genotype polymorphism of LOX-1 3′-UTR ＋ 1073 is not closely associated with CHD and it is not the hereditary susceptible sign of CHD.