菜豆黄花叶病毒中国和叙利亚蚕豆分离物外壳蛋白的序列分析(英文)

Sequence Analysis of the Coat Protein Gene of Bean yellow mosaic virus Isolates from Faba bean in Yunnan,China and Syria

病毒病是影响云南省蚕豆生产的重要病害。对采集的蚕豆病毒病标样进行了组织印迹法检测,表明菜豆黄花叶病毒(BYMV)是最主要的病原。据此,以BYMV基因的保守序列设计了一对特异性引物,用BYMV的5个中国云南蚕豆分离物和1个叙利亚蚕豆分离物侵染的蚕豆叶片总RNA为模板,RT-PCR扩增获得了长度为907bp的目标片段。序列分析显示,此片段中包含822bp的外壳蛋白序列。6个分离物间的外壳蛋白核苷酸和推导编码蛋白质的氨基酸序列的同源性分别为86.4%～100.0%和96.7%～100.0%。与GenBank登录的34个具有完整外壳蛋白序列的BYMV分离物进行同源性和系统进化树分析的结果表明,6个分离物在核苷酸和氨基酸水平上与其它分离物的同源性分别为79.1%～97.9%和83.5%～98.5%,BYMV中国蚕豆分离物与日本蚕豆分离物同源性最高。外壳蛋白基因的序列特征揭示,在BYMV中的蚜传相关基序为NAG。

Viral diseases are one of the major factors for yield loss of faba bean in Yunnan province, China. Bean yellow mosaic virus （BYMV） was identified in faba bean samples by Tissue Blot Immunoassay （TBIA）. According to BYMV sequence submitted to GenBank, a pair of primer was designed and used to amplify the coat protein （CP） gene of BYMV in six faba bean isolates, five from Yunnan province and one from Syria, by reverse transcription PCR （RT-PCR）. The amplified fragment was cloned and in which 822 nucleotides was for CP gene. The CP gene was found to be 86.4% to 100.0% and 96.7% to 100.0% homologous among six BYMV isolates at both the nucleotide and amino acid level. The CP gene of six sequenced isolates showed 79.1% to 97.9% and 83.5% to 98.5% nucleotide and amino acid identifies with 34 BYMV isolates in GenBank. The comparison data of CP gene sequences showed that BYMV-Chinese faba bean isolates were closely related to the BYMV-Japanese faba bean isolates. The NAG motif, a basic structure for aphid transmission of the virus located at the N-terminus of CP gene in BYMV, was presented in all of six isolates.