摘要
Using resistant and susceptible cultivars as controls,the resistant evaluation to Pseudoperonospora cubensis was carried out in 12 introgression lines from wide cross between cucumber(Cucumis sativus L.)and sour cucumber(Cucumis hystrix Chakr.).Three enzyme activities including POD,SOD and PAL were analyzed before and 7 d after inoculation,and the POD isozyme was detected by polyacrylamide electrophoresis.The inoculation results showed that,of the 12 accessions,3 were identified as high resistant,5 were moderately resistant and 4 were moderately susceptible to downy mildew.The enzyme activities of POD,SOD and PAL were greatly increased in resistant accessions after inoculation.PAL enzyme activities showed close correlation with disease rating before or after inoculation,which implicated that PAL enzyme activity might be used to estimate the resistance to downy mildew.POD isozyme electrophoresis showed that the number and intensity for the bands of resistant lines were significantly increased more than those of susceptible lines after inoculation.
Using resistant and susceptible cultivars as controls, the resistant evaluation to Pseudoperonospora cubensis was carded out in 12 introgression lines from wide cross between cucumber (Cucumis sativus L. ) and sour cucumber (Cucumis hystrix Chakr. ). Three enzyme activities including POD, SOD and PAL were analyzed before and 7 d after inoculation, and the POD isozyme was detected by polyacrylamide electrophoresis. The inoculation results showed that, of the 12 accessions, 3 were identified as high resistant, 5 were moderately resistant and 4 were moderately susceptible to downy mildew. The enzyme activities of POD, SOD and PAL were greatly increased in resistant accessions after inoculation. PAL enzyme activities showed close correlation with disease rating before or after inoculation, which implicated that PAL enzyme activity might be used to estimate the resistance to downy mildew. POD isozyme electrophoresis showed that the number and intensity for the bands of resistant lines were significantly increased more than those of susceptible lines after inoculation.
出处
《植物病理学报》
CAS
CSCD
北大核心
2007年第4期433-437,共5页
Acta Phytopathologica Sinica
基金
国家高技术研究发展计划专项经费资助(2006AA10Z1A8
2006AA100108)
国家自然科学基金资助(30470120
30671419)
江苏高校高新技术产业发展项目资助(JHB04-012)
PSRFofPPI
国家科技支撑计划(2006BAD13B06)
关键词
黄瓜-酸黄瓜渐渗系
霜霉病
抗性
酶活性
霜霉病菌
introgression lines
downy mildew
enzyme activity
POD isozyme electrophoresis