马铃薯疮痂病菌致病相关基因的克隆及表达

Cloning and expression of a pathogenic-related gene in Streptomyces scabies CPS-1

A pathogenic-related gene nec1 was cloned in Streptomyces scabies CPS-1,potato scab strain.Analysis results showed that the length of open reading frame(ORF)for nec1 gene was 666 bp,and the GC content was 54.2%.Sequence alignment indicated that a 650 bp up-stream sequence shared 91% similarity with IS 256 family transposase nucleotide sequences by BLASTn searches against GenBank.The segments obtained by PCR amplification were digested by enzymes SphⅠand SacⅠ,and linked to the expression vector pIJ702.The recombinants were transformed into nonpathogenicity strain Streptomyces lividans 66 TK24.Bioas-say results suggested that the transformants possessed the same symptoms as pathogenic strain on potato tuber slices and radish seedlings,which implied that nec1 gene was associated with the pathogenicity of S.scabies CPS-1.

A pathogenic-related gene necl was cloned in Streptomyces scabies CPS-I, potato scab strain. Analysis results showed that the length of open reading frame （ORF） for necl gene was 666 bp, and the GC content was 54.2%. Sequence alignment indicated that a 650 bp up-stream sequence shared 91% similarity with IS 256 family transposase nucleotide sequences by BLASTn searches against GenBank. The segments obtained by PCR amplification were digested by enzymes Sph Ⅰ and Sac Ⅰ , and linked to the expression vector pIJ702. The recombinants were transformed into nonpathogenicity strain Streptomyces lividans 66 TK24. Bioassay results suggested that the transformants possessed the same symptoms as pathogenic strain on potato tuber slices and radish seedlings, which implied that necl gene was associated with the pathogenicity of S. scabies CPS-1.