MPOmRNA、IgHmRNA和CD_3εmRNA表达对急性白血病分型的意义

The role of MPO mRNA,IgH mRNA and CD 3ε mRNA expressions in classification of acute leukemias

目的：比较基因分型和免疫分型在急性白血病诊断分型中的意义。方法：采用链亲和素－胶体金细胞原位杂交技术（ＩＳＨ－ＳＡＧ），研究了６９例急性白血病的髓过氧化物酶（ＭＰＯ）ｍＲＮＡ、免疫球蛋白重链（ＩｇＨ）ｍＲＮＡ和ＣＤ３εｍＲＮＡ的基因表达并同时分析了白血病的免疫表型。结果和结论：ＭＰＯｍＲＮＡ和ＣＤ３εｍＲＮＡ敏感性高、特异性强，对确诊早期ＡＭＬ和Ｔ－ＡＬＬ有重要意义，ＩｇＨｍＲＮＡ则对识别更早期Ｂ细胞白血病有一定作用。用ＩＳＨ－ＳＡＧ进行基因分型是个新的更加敏感、特异的白血病诊断分型手段，将基因分型和免疫分型结合分析，对白血病更精确的诊断分型有重要意义。

Objective:To compare the role of genotypic and immunophenotypic markers in classification of acute leukemia.Methods:Expression of myeloperoxidase(MPO),CD 3ε chain and immunoglobulin heavy chain(IgH) gene in 69 cases of acute leukemia were studied by in situ hybridization with streptavidin gold (ISH SAG).Results and Conclusion:MPO mRNA and CD 3ε mRNA expressions were specific for early diagnosis of AML and T ALL,respectively;and IgH mRNA expression did for early B cell leukemia.Combination of genotypic and immunophenotypic markers assured accurate lineage diagnosis of leukemias.