雪旺细胞和层粘蛋白对脊髓前角运动神经元损伤的保护作用

PROTECTIVE EFFECT OF SCHWANN CELLS AND LAMININ ON SPINAL MOTONEURONS BY SCIATIC NERVE RESECTION

选30只成年Wistar大鼠,坐骨神经切断后,分别应用体外培养的雪旺细胞、层粘蛋白和生理盐水于神经近侧断端,4周后,观察损伤侧腰4、5节段脊髓前角运动神经元的存活率;神经元酸性磷酸酶和胆碱脂酶活性变化.结果:生理盐水组脊髓前角运动神经元存活率为59%,酸性磷酸酶活性明显增强,胆碱脂酶活性明显降低;雪旺细胞组和层粘蛋白组脊髓前角运动神经元存活率分别为82.3%和81.1%;酸性磷酸酶和胆碱脂酶活性较对照组分别减弱和增强.提示雪旺细胞和层粘蛋白对受损的脊髓前角运动神经元具有明显的保护作用,有利于神经再生.

The present study was made to investigate the protective effect of Schwann cells (SO and laminin (LN) on the motor neurons of the anterior horn of spinal cord. The sciatic nerves of 30 adult Wistar rats were transected. The results were as follows: 41% of the spinal motor neurons died 4 weeks after transection of the sciatic nerve. The survival rate of the spinal motor neurons cultured with SC or LN increased to 82. 3% or 81. 2%. The results showed that SC and LN decreased the acid phosphatase(ACP) activity and increased the cholinesterase(ChE) in the injuried motor neurons. It can be concluded that the protective effect of SC and LN on the motor neurons plays an important role in peripherial nerve regeneration.