壳聚糖-对羟基苯甲酸酶法接枝及表征

Enzymatic Grafting and Characterization of Chitosan-(p-Hydroxybenzoic Acid)

从土豆中提取酪氨酸酶（tyr），以该酶催化氧化对羟基苯甲酸（BA）成活性邻醌，成功地实现了壳聚糖（Cs）3z的接枝。通过UV-Vis、FTIR、TG-SDTA对其反应产物进行结构和性能的初步表征，由线性电位滴定法测得其接枝率为47％～58％。

Tyrosinase was extracted from potatoes .The 4-hydroxybenzoic acid was catalyzed and oxidized by tyrosinase to reactive o-quinones, which were grafted onto chitosan. The structure and property of the grafted product were characterized by UV-Vis ,FTIR and TG-SDTA. The results indicated that the 4-hydroxybenzoic acid had been grafted onto chitosan film by tyrosinase.The graft rate was determined to be 47% - 58% with linear titrimetric method.