口蹄疫病毒Asia1/YNBS/58株全基因组序列分析

Genome Sequencing and Analysis of Foot-and-mouth Disease Virus Asia1/YNBS/58 Strain

The full-length genomic sequence of foot-and-mouth disease virus(FMDV) Asia1/YNBS/58 strain was determined by RT-PCR and compared with other 17 reference strains.The results showed that the complete genome of Asial/YNBS/58 was 8164nt long including a 1061-nt 5′untranslated region(UTR),a 6990-nt open reading frame(ORF),and a 113-nt 3′UTR.The homology analysis indicated that the UTR regions and non-structural proteins were more conserved than the structural proteins in FMDV.VP1 exhibited the lowest conservation and VP4 was exceptionally conserved.The VP1-,VP2-,and VP3-based phylogenetic trees were divided into distinct clusters according to different serotypes,while the other gene-based phylogenetic trees exhibited some degree of intercross among serotypes.This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.

The full-length genomic sequence of foot-and-mouth disease virus （FMDV） Asia1/YNBS/58 strain was determined by RT-PCR and compared with other 17 reference strains. The results showed that the complete genome of Asia1/YNBS/58 was 8164nt long including a 1061-nt 5＇ untranslated region （UTR）, a 6990-nt open reading frame （ORF）, and a 113-nt 3＇UTR. The homology analysis indicated that the UTR regions and non-structural proteins were more conserved than the structural proteins in FMDV. VP1 exhibited the lowest conservation and VP4 was exceptionally conserved. The VP1-, VP2-, and VP3- based phylogenetic trees were divided into distinct clusters according to different serotypes, while the other gene-based phylogenetic trees exhibited some degree of intercross among serotypes. This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.