摘要
选用荷兰百合品种索尔邦鳞茎、顶芽、茎段、叶等不同部位进行离体,以鳞茎为最佳外植体,以MS+6-BA 2.0 mg/L+IAA 1.0 mg/L的培养基诱导丛生芽效果最好,其诱导率为96.7%,而且鳞片诱导中鳞茎中部表现出较大的器官发生潜力。百合继代增殖培养以MS+BA1.0 mg/L+IAA 0.4 mg/L培养基为宜,组培苗在MS+IAA 0.5 mg/L的培养基中较易生根,生根时间为25 d左右。
The experiment selects Holland Lily variety Sorbonne bulb, the terminal bud, the stem section, the leaf and so on, as is best, by MS ＋6-BA 2. 0 mg/L＋IAA 1.0 mg/L culture medium induction grows thickly, the bud effect to be best, its inductivity is 96. 7%, and in the scales induction, The central section displays the big organogenesis potential.The lily continues the generation of multiplication raise by MS＋BA 1.0 mg/L＋IAA 0. 4 mg/L for is suitable, the tissue culture plant in MS ＋IAA0. 5mg/L is easy to take root, about 25 days.
出处
《北方园艺》
CAS
北大核心
2007年第9期191-192,共2页
Northern Horticulture
基金
中国工程物理研究院横向合作研究资助项目(2003205)
