藿香植物组织培养技术研究

Study on the Plant Tissue Culture Technology of Agastache rugasa(Fisch.et Meyer) O.Ktze

选用藿香健壮枝条和叶片作为试验材料，进行组培技术的研究。结果表明：藿香取材季节以夏季较好，适宜的外植体种类为茎段。诱导愈伤组织形成的激素组合为6-BA1．0mg／L＋NAA0．3mg／L＋2，4-D0．3mg／L，具有愈伤组织生长速度快并易于分化；不定芽分化激素组合为6-BA0．5mg／L＋NAA0．1mg／L，继代培养组合6-BA0．1mg／L＋NAA0．5mg／L＋B9 0．3mg／L，增殖在8倍以上；生根培养组合1／2MS＋NAA0．6mg／L，一般20d左右生根，平均根量4～5条，生根率达92％。

Selectedthe vigorous and healthy branch and leaf of Agastache rugasa （Fisch. et Meyer） O. Ktze as explants carried on the plant tissue culture technology research. The results showed that summer was the best season for plant tissue culture,plant hormone combination of 6-BA 1.0 mg/L ＋ NAA O. 3 mg/L＋2,4-D 0. 3 mg/L was good for callus induction, 6-BA 0. 5 mg/L＋NAA 0. 1 mg/L was suitable for Adventitious bud formation, 6-BA 0. 1 mg/L＋ NAA 0.5 mg/L ＋ B9 0. 3mg/L was good for cultivation of young plant, the coefficient of reproduction was up to 8 times. NAA0. 6 mg/L was bestfor rootage, rooting rate was 92%.