巨桉优良无性系组培及区域试验

The tissue culture and regional tests of excellent clones of Eucalyptus grandis

通过对巨桉11个优良无性系组培快繁研究,结果表明,巨桉外植体诱导以B1为基本培养基添加激素BA 0.4 mg.L-1和NAA 0.1～0.5 mg.L-1,诱导外植体最佳,诱导率可达79%以上;丛芽苗增殖调节培养基中激素浓度和基本培养中N、P、K、Ca、Mg、Fe的浓度配比使其芽体健壮,加快繁殖速度;生根培养以B2为基本培养基添加ABT 0.5 mg.L-1+IBA0.1～0.5 mg.L-1生根率可达80%以上;组培苗移栽基质选用泥炭土和竹炭肥,成活率达90%以上。区域试验表明:巨桉是较理想的速生耐寒桉树树种,可耐寒-4℃左右。

The tissue culture and rapid propagation of 11 excellent clones of Eucalyptus grandis were studied in this paper. The results showed the optimal explant induction medium was B1 supplemented with 0.4 mg· L^-1 BA and 0.1 - 0.5 mg·L^-1 NAA, and its induction rate was more than 79 %. In the cluster bud propagation medium, the proper combination of hormones and N, P, K, Ca, Mg, Fe concentration made the buds and shoots strong, and propagation quickly. The optimal rooting medium was 132 supplemented with 0.5 mg·L^- 1 ABT and 0.1- 0.5 mg·L^- 1 IBA, and its rooting rate could achieve 80 %. The soil medium for transplant was peat moss and bamboo charcoal fertilizer, its survival rate reached more than 90 %. The regional tests indicated that Eucalyptus grandis was the ideal fast-growth and cold-tolerance species which could bear - 4℃.