耐冬山茶愈伤组织诱导研究

The preliminary studies on the callus induction of Camellia japonica "Naidong"

重点探讨耐冬山茶愈伤组织诱导过程中不同植物生长调节剂、培养基pH值、不同培养基及不同类型外植体对愈伤组织诱导效应的影响。研究结果表明:MS+6-BA 2.0 mg.L-1+2,4-D 0.5 mg.L-1培养基是耐冬山茶愈伤组织诱导的最佳培养基;耐冬山茶愈伤组织诱导培养基pH值以5.5较为合适;耐冬山茶叶柄作为外植体,诱导率和生长状况最佳。

The experiment is to study the callus induction effect by using different kinds of hormones, different mediums, pH and different explants, in order to develop the technique systems of tissue culture and rapid propagation for C. japonica ＂Naidong＂. The major results as the following： Medium MS ＋ 6-BA 2.0 mg·L^-1 ＋ 2, 4-D 0.5 mg·L^ - 1 is optimum for callus induction of C. japonica ＂Naidong＂ ;The pH 5.5 is comparatively suitable for C. japonica ＂Naidong＂ callus induction; Using the leafstalk of C. japonica ＂Naidong＂ as the explants, the induction rate and the growth is best