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共刺激分子配体B7-DC转基因小鼠模型的建立

Establisment of B7-DC Transgenic Mouse
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摘要 目的建立共刺激分子配体B7-DC转基因小鼠模型。方法利用RT-PCR方法从来自小鼠胸腺的mRNA中扩增出B7-DC cDNA,继而将B7-DC基因插入到真核表达载体PEF6/V5-His中得到含B7-DC和V5-His融合蛋白的PEF6-B7-DC/V5-His。注射外源基因PEF6-B7-DC/V5-His至FVB小鼠原核,注射胚胎移植到同期发情的假孕受体,所生仔鼠经PCR和Southem-blot检测获得阳性转基因小鼠。结果共注射移植胚胎87枚,出生小鼠39只,检测出阳性小鼠3只,并稳定遗传至F2代。结论成功建立了B7-DC转基因小鼠模型。 Objective Establishment of B7-DC transgenic mouse model. Method B7-DC cDNA was cloned by RT-PCR from mouse thymus, then it was inserted into PEF6/V5-His vector and get PEF6- B7-DC/V5-His which contain a fuse protein B7-DC-V5-His. The PEF6-B7-DC/V5-His gene was micro- injected to the pronuclear of FVB mouse embryos, then transfer the embryos to the foster mother and get new individuals. Positive founders was confirmed by PCR and Southern. Result Eighty three injected embryos were transferred and 39 offspring mice were obtained, three of them are positive and can be successfully inherited to F2. Conclusion The BT-DC transgenic mice model was established.
作者 刘英 岳挺 施云松 袁文越
机构地区 上海医药工业研究院药理室 扬州大学比较医学中心 上海瑞集医学科技有限公司
出处 《实验动物与比较医学》 CAS 2007年第3期176-179,共4页 Laboratory Animal and Comparative Medicine
关键词 B7-DC 转基因 动物模型 B7-DC Transgenic Animal model
分类号 R-332 [医药卫生]
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参考文献7

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二级参考文献30

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实验动物与比较医学
2007年 第3期

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