高压氧治疗对脑损伤大鼠脑组织中白细胞介素-1β和肿瘤坏死因子-α含量的影响

Effects of hyperbaric oxygen on the content of interleukin-1β and tumor necrosis factor-α in brain injured tissue of rats

目的观察重度侧位液压撞击脑损伤SD大鼠在高压氧治疗前后损伤灶局部脑组织中白细胞介素(IL)-1β和肿瘤坏死因子(TNF)-α的含量变化,以探讨高压氧治疗创伤性脑损伤可能的作用机制。方法应用液压撞击仪建立重度创伤性脑损伤模型,用ELISA方法检测脑组织匀浆中IL-1β和TNF-α的含量。结果高压氧治疗后脑组织匀浆中IL-1β和TNF-α含量低于相应时间点损伤组,差异有统计学意义(P<0.05)。结论高压氧治疗可能通过抑制IL-1β和TNF-α等炎症介质的表达,减轻创伤性脑损伤后的炎症反应。

Objective To observe the changes of content of interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） before and after hyperbaric oxygen （HBO） treatment in brain injured tissue of SD rats following severe lateral fluid-percussive brain injury and to explore the mechanism of HBO treatment on traumatic brain injury. Methods Severe traumatic brain injury models were established by the fluid percussion device. The concentrations of IL-1β and TNF-α in the homogenate of brain tissue were measured by using enzyme-linked immunosorbent assay （ELISA） respectively. Results The concentration of IL-1β and TNF-α in brain tissue homogenate in the HBO group was significantly lower than that of the traumatic brain injury group （P 〈 0.05 ）. Conclusions HBO treatment can effectively relieve the inflammatory reaction of brain injured tissue in rats through inhibiting the expression of IL-1β and TNF-α.