水鲨头皮胶原蛋白分离及其部分特性研究

Study of the characterization and isolation of collagen from blue shark scalp

目的从水鲨头皮分离得到胶原蛋白并研究其部分生化特性。方法水鲨头皮用不同浓度的氢氧化钠溶胀,去离子水清洗至中性,经胃蛋白酶限制性酶解,离心,上清液经透析后冷冻干燥,得到胶原蛋白制品。用聚丙烯酰胺凝胶电泳、差式扫描量热仪(DSC)、圆二色谱仪等研究了分离得到的胶原蛋白的特性。结果胶原蛋白的回收率为鲜重的2.9%～4.1%;在十二烷基硫酸钠聚丙烯酰胺凝胶电脉(SDS-PAGE)上呈现清晰的3条谱带,相对分子质量分别为205 000、134 000、118 000,热变性温度为69℃。结论分离得到的胶原蛋白可进一步用于微囊剂、纳米材料等生化材料的研究。

Objective To study the isolation of collagen from the blue shark scalp and its some biochemical properties. Methods The collagen was purified from blue shark by soaking in the alkali solution, limit-digesting in pepsin solution, dialyzing , centrifugation and lyophillization. The measurements of some biochemical properties were determined with SDS-PAGE, DSC and circular dichroism. Results Yield of the collagen was 2.9% -4. 1% in original skins. The collagen was detected three chains （205 000,134 000,118 000） with SDS-PAGE,and its maximal thermal denature temperature was 69℃. Conclusions These findings suggest that the collagen obtained by above method can be used for further reaches of biochemical properties, such as the nano-materials, tiny-capsules and so on.