摘要
利用荧光和紫外光谱法研究了培氟沙星（PEF）-Al^3＋配合物与DNA的相互作用。培氟沙星（PEF）能与Al^3＋生成PEF-Al（Ⅲ）二元配合物，该配合物能与DNA发生相互作用。利用DNA与PEF-Al（Ⅲ）作用能猝灭体系荧光强度的性质测定DNA，其线性范围为8．0×10^-6～2．25×10^-4 mol／L，检出限为5．0×10^-6mol／L。讨论了反应的最佳条件及其结合机理。
The interaction of pefloxacin （PEF）-AI（ Ⅲ ） complex with DNA was studied by fluorescence and UV spectrametric methods. The spectral change in the presence of aluminum ions showed that the PEF-Al binary complex was formed and that the complex could interact with DNA. DNA can also be determined by the interaction of PEF-Al binary complex with DNA in the range from 8.0×10^-6 to 2.25×10^-4 mol/L with the detection limit of 5.0 ×10^-6 mol/L. The optimum reaction conditions and the mechanism of the formation were also discussed.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第9期70-73,共4页
Chinese Journal of Analysis Laboratory
