实验性糖尿病大鼠动脉粥样硬化模型的建立

Experimental Rat Model of Diabetes Mellitus with Arteriosclerosis

目的建立类似人类糖尿病并发动脉粥样硬化(AS)大鼠模型。方法选1月龄SD大鼠,先饲以高脂高糖饲料4周,促发胰岛素抵抗,继以小剂量STZ(30mg/kg·体重)腹腔注射,诱导建立糖尿病AS模型。结果注射STZ2周后大鼠血糖、甘油三酯、高密度脂蛋白、低密度脂蛋白及HDL-L/总胆固醇均显著高于空白组;形态学观察发现,模型组动脉出现典型粥样硬化病变。结论本实验方法建立的糖尿病伴动脉粥样病变大鼠模型,与人类2型糖尿病代谢特征相似,是研究人类糖尿病大血管病变较理想的动物模型。

] Objective：To estable rat model of diabetes mellitus with arteriosclerosis that simulates the metabolic abnormalities of human disease. Methods： 1 month old Sprague Dawley rats were fed on a diet enriched in fat and glucose to induce insulin resistance , after 4 weeks, the rats were injected intrapertoneally with a low - dose streptozotocin （STZ） （30mg/kg·BW）to induce hyperglycemia with arteriosclerosis. Results： After the 2nd STZ injection, the rats demonstrated the blood glucose, TG, HDL - L, LDL - L and HDL - L/TC levels were significantly risen up . Moreover, the significant pathological changes of arteriosclerosis appeared in the model rats＇ arteries after 11 weeks. So the successful model was made. Conclusion： This model closely simulates the human diabetes mellitus with arteriosclerosis, this study provides the ideal animal model for investigating human diabctes with macroangiopathy.