多价不饱和脂肪酸发醇条件的初步研究

Studies on Fermentation Condition of Polyunsaturated Fatty Acids

研究被孢霉SD－95的摇瓶发酵特性，选出最优培养基配方。摇瓶发酵结果为，菌体得率28．5％，油脂得率12．4％，油脂含量43．6％，碘值122．8。油脂中多价不饱和脂肪酸组成为，亚油酸17．2％，r-亚麻酸7．7％，花生四烯酸0．5％，多价不饱和脂肪酸总量为25．3％；菌丝体经过冬化处理后，其多价不饱和脂肪酸组成为，亚油酸18．6％，r-亚麻酸8．1％，花生四烯酸0．7％，二十碳五烯酸50．4mg／1009油脂，多价不饱和脂肪酸总量27．5％。

Some studies on the fermentation condition of the Mortierella SD-95 weremade. The optimum culture medium was obtained through orthogonal designs,and the resultof fermentation showed that the production rate of biomass was 28. 5%,the production rateof lipid was 12. 4%, lipid content was 43. 6%, lV was 122. 8. A gas chromatography testshowed that the content of linoleic acid (LA) was 17. 2% in the fatty acids of the lipids ob-tained,the content of Y-linolenic acid(GLA) was 7. 7%,the content of arachidonic acid(AA)was 0. 5%,all the content of PUFA was 25. 3%. After mycellium was dealt with low tem-perature,the content of GLA was 8. 1 %,the content of AA was 0. 7%,the content of all-cis-5, 8, 11, 14, 17-eicosapentagenoic acid (EPA)was 50mg/100g lipid,all the content of PUFAwas 27. 5 %.