摘要
对胆红素氧化酶产生菌的菌和选育进行了研究。以科研室保藏菌种Mv9201为出发菌株,通过紫外线、硫酸二乙酯单独处理和复合诱变,从353株突变株中筛选出一株高产菌株MyrotheciumverrucariaMv9352,并对其产酶条件进行了研究。以20%的土豆汁(含0.25%的葡萄糖和蛋白胨)为发酵培养基,初始pH6~7,摇床转速为140~160r/min,24~26℃培养96h,酶活力可达4.0~4.5u/ml,比出发株的产酶能力提高近4.5倍。
?A mutant strain Mv9352 with high yeild of bilirubin oxidase (EC1. 3. 3. 5)was abtained from 353 mutants of Myrothecium vermcaria Mv9201 by treatments of UV,DES and DES-UV. The optimum conditions of enzyme production were investigated and re-sults as follows: the suitable medium was 20% potato decoction including 0. 25% glucose and0. 25% peptone,initial pH 6~7. The strain was incubated at 24~26℃ on a rotating shaker(140~160r/min) for 96h. The enzyme activity was improved from 1. 0 u/ml to 4. 0~4.5 u/ml.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
1997年第2期22-25,33,共5页
Food and Fermentation Industries
基金
北京市自然科学基金