胆红素氧化酶产生菌的选育及发酵工艺条件的研究

Breeding of Bilirubin Oxidase Producing Strain and the Optimum Conditions of Enzyme Production

对胆红素氧化酶产生菌的菌和选育进行了研究。以科研室保藏菌种Mv9201为出发菌株，通过紫外线、硫酸二乙酯单独处理和复合诱变，从353株突变株中筛选出一株高产菌株MyrotheciumverrucariaMv9352，并对其产酶条件进行了研究。以20％的土豆汁（含0．25％的葡萄糖和蛋白胨）为发酵培养基，初始pH6～7，摇床转速为140～160r／min，24～26℃培养96h，酶活力可达4．0～4．5u／ml，比出发株的产酶能力提高近4．5倍。

?A mutant strain Mv9352 with high yeild of bilirubin oxidase (EC1. 3. 3. 5)was abtained from 353 mutants of Myrothecium vermcaria Mv9201 by treatments of UV,DES and DES-UV. The optimum conditions of enzyme production were investigated and re-sults as follows: the suitable medium was 20% potato decoction including 0. 25% glucose and0. 25% peptone,initial pH 6～7. The strain was incubated at 24～26℃ on a rotating shaker(140～160r/min) for 96h. The enzyme activity was improved from 1. 0 u/ml to 4. 0～4.5 u/ml.