小鼠转移性乳腺癌骨髓细胞体外净化移植模型的建立

Establishment and application of a murine transplant model of bone marrow purging of metastatic breast cancer cells in vitro

目的建立一个小鼠转移性乳腺癌净化后骨髓移植模型,并探讨益康唑(Econazole,Ec)药物净化效果。方法将小鼠自发性乳腺癌细胞系 TSA 转染新霉素抗性基因(Neo^R 基因)后与小鼠骨髓细胞混合,体外短期培养净化;通过移植后造血恢复,肺转移性结节和小鼠的存活进行评估。结果接受放射治疗后经尾静脉注射 TSA 细胞的小鼠均可产生乳腺癌肺转移;Ec 体外净化后骨髓移植小鼠造血恢复没有明显延迟,移植后4周内未见肺转移性结节;小鼠的总生存状况明显改善。结论成功建立了 TSA 小鼠乳腺癌净化后骨髓移植模型,方法简单、易行、重复性好;Ec 可用于转移性乳腺癌净化后骨髓移植。

Objective To establish a murine transplant model for bone marrow purging of metastatic breast cancer and to explore the efficiency of Econazole （Ec） as a purging agent. Methods Mixtures of TSA /NeoR breast cancer cells and murine bone marrow cells were transplanted into lethally irradiated mice following purging with Econazole or saline in vitro. The recipient mice were monitored for hematopoietic engraftment, appearance of metastatic nodules in lungs and the overall survival. Results All the mice receiving i.v. injection of TSA cells developed metastatic lung nodules. The hematological recovery was not delayed in mice transplanted with Ec purged bone marrow. More importantly, metastatic lung nodules were not seen in Ec treated group and the overall survival was improved. Conclusion The purged metastatic breast cancer cell bone marrow transplant model was easily established and reproducible. Ec could be used to purge the bone marrow grafts contaminated with breast cancer cells.