免疫性肝损伤小鼠外周血淋巴细胞亚群变化及大蒜多糖C的干预作用

Changes of Lymphocyte Subsets in the Peripheral Blood of Mice with Immunologial Liver Injury and Intervention of Garlic Polysaccharide C

目的观察免疫性肝损伤小鼠外周血淋巴细胞亚群变化并研究大蒜多糖C的干预作用。方法采用尾静脉注射卡介苗加脂多糖(BCG+LPS)建立免疫性肝损伤模型,应用流式细胞术测量小鼠外周血淋巴细胞亚群的百分比和CD_4^+/ CD_8^+比值。结果模型组T淋巴细胞、CD_8^+T细胞百分比值较正常对照组明显升高(P<0.01),B淋巴细胞百分比值、CD_4^+/CD_8^+比值明显下降(P<0.01),CD_4^+T细胞百分比也有减少(P<0.05),NK1.1^+T细胞百分比无显著变化。大蒜多糖C(800 mg/kg ig,qd×12)治疗能使BCG+LPS免疫性肝损伤小鼠外周血T,B.CD_4^+T和CD_8^+T淋巴细胞百分比显著下降,而CD_4^+/CD_8^+比值显著上升,恢复到正常水平,对NK1.1^+T细胞百分含量则无明显影响。结论大蒜多糖C能全面抑制免疫性肝损伤小鼠的免疫功能,并促使机体建立新的免疫平衡,从而对免疫性肝损伤产生保护作用。

Objective To observe the changes of lymphocyte subsets in the peripheral blood of mice with immunological liver injury and intervention of Garlic Polysaccharide C. Methods The animal immunological liver damage model was reproduced by injection via the tail vein of BCC （Bacilli Calmette Cuein）and lipopolysaccharide（LPS） into mice. The peripheral -blood lympho- cyte subsets＇percentages and the CD4 ^＋/CD8^＋ ratio was measured by the flow cytometry. Results Compared with normal control group, the percentages of the T lymphocyte and the CD8^ ＋ T lymphocyte in the peripheral - blood in immunological liver damage mice siginificantly increased （P 〈 0.01 ）, however, the percentages of the B lymphocyte, CD4^ ＋ T lymphocyte and the ratio of CD4^ ＋/CD8^ ＋ decreased（ P 〈 0.05 or P 〈 0.01 ）. Garlic polysaccharide C at dose of 800 mg/kg （ ig, qd x 12） decreased the percentages of T,B,CD4^ ＋ T and CD8 ^＋ T lymphocyte in the peripheral - blood in mice treated with BCG ^＋ LPS, however,the CD4^ ＋/ CD8^ ＋ ratio increased to the normal level. The percentages of the NK1.1^ ＋ T lymphocyte showed no obvious difference between control, BCC^ ＋ LPS liver injury model and Garlic Polysaccharide C groups. Conclusion Garlic Polysaccharide C can inhibit the immunologic activity, and establish a new immunologic balance, thereby produce protective effects on immunological liver injury.