摘要
目的建立三七超微粉的质量标准。方法采用薄层色谱法鉴别三七;使用激光粒度检测仪对粒径进行检测;HPLC法测定三七超微粉中三七皂苷R_1,人参皂苷Rg_1,人参皂苷Rb_1含量。结果薄层图谱斑点清晰,可鉴别出与三七的对应斑点;三七超微粉的中位径在15μm以下;三七皂苷R_1在0.535~3.210μg范围内线性关系良好(r=1.000),平均回收率103.08%,RSD=2.80%;人参皂苷Rg_1在2.225~13.350μg范围内线性关系良好(r=1.000),平均回收率100.33%,RSD=2.29%;人参皂苷Rb_1在2.205~13.230μg范围内线性关系良好(r=1.000),平均回收率101.00%,RSD=1.43%%。结论所建立的方法简便、准确、重复性好,可用于三七超微粉的质量控制。
Objective To develop a quality standard for super micropowder of notoginseng. Methods Qualitative analysis of micropowder of notoginseng was carried out by TLC. The particle diameter was detected with laser scattering particle size distribution anaslyzer. An RP - HPLC method was used for the content determination of the Notoginsensode R1 , ginsenoside RgI , ginsen- oside RbI. Results The chromatographic spots of micropowder of notoginseng were identified without the interference of negative control. Micropowder of particle average diameter was below 15bLm. Notoginsensode R1 had a good linearity in the range of 0. 535 - 3. 210 bLg ( r = 1. 000) ,and the average recovery was 103.08% with RSD of 2.80%. Ginsenoside Rg1 had a good linearity in the range of 2. 225 - 13. 350 bLg (r = 1. 000) ,and the average recovery was 100.33 % with RSD of 2.29 %. Ginsenoside Rb1 had a good linearity in the range of 2. 205 ~ 13. 230 bLg ( r = 1. 000 ) , and the average recovery was 101.00% with RSD of 1.43%. Conclusion This method is simple , accurate and repeatable. It can be used to determine ginsenoside Rgl, ginsenoside RbI and notoginsensode R1 in micropowder of Notoginseng.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第9期2159-2161,共3页
Lishizhen Medicine and Materia Medica Research
基金
四川省科技厅应用基础研究资助项目(No.2006-B-KJT-02)
关键词
三七超微粉
粒径
高效液相
薄层色谱
质量标准
Micropowder of Notoginseng
Particle diameter
HPLC
TLC
Quality standard