黑曲霉诱导子促进岩黄连悬浮细胞中生物碱的合成

Enhancement of Alkaloid Biosynthesis by A Elicitor of Aspergillus niger in Cell Suspension Cultures of Corydalis saxicola Bunting

目的为了提高岩黄连悬浮培养细胞中生物碱的产量。方法以不同浓度黑曲霉诱导子处理岩黄连悬浮培养细胞。结果生物碱的产量随着诱导时间的延长迅速提高,到第6天达到最大值;添加50mg/L诱导子时生物碱产量最高,达到89.2mg/L,比时照组高出2.5倍,当诱导子浓度进一步升高时,生物碱产量反而降低;随着诱导子浓度从0mg/L增大到150mg/L,细胞生物量反而逐渐减少。结论黑曲霉诱导子可促进岩黄连细胞中生物碱的合成,最佳诱导子浓度是50mg/L;过高浓度的诱导子并不利于细胞生长和生物碱合成,细胞生物量的减少是导致生物碱产量下降的一个重要原因。该处理提供了一种简单有效的方法提高岩黄连细胞培养中生物碱的产量。

Objective To improve the production of alkaloids in Corydalis saxicola Bunting suspension cell cultures. Methods Elicitor prepared from AspergiUus niger was used to treat the suspension cells on day 12. Results The results showed that the production of alkaloids increased with the extension of time after treatment and the greatest value of alkaloid production was obtained on day 6. Cultures treated with 50 mg/L elicitor generated the highest alkaloid production, 89.2 mg,/L, which was about 2.5 - fold of that in the control. Alkaloid production significantly decreased in response to increased elicitor concentration from 50 mg/L to 150 mg/L. When elicitor concentration increased from 0 to 150 mg/L, biomass of cultures inversely decreased. Conclusion This elicitor is effective to enhance alkaloid biosynthesis in cell suspension cultures of C. saxicola and the optimal concentration for alkaloid biosynthesis is 50 mg/L. Higher concentration of the elicitor is unfavorable to not only cell growth but also alkaloid production. This decrease of biomass induced by higher concentration of elicitor is a main reason for the suppression of alkaloid production. This treatment provides a simple and effective approach to enhance alkaloid biosynthesis of C. saxicola cells.