冠心病患者内皮脂肪酶基因Thr111Ile和Gly26Ser多态性与脂蛋白代谢的关系

Association of endothelial lipase gene Thr111Ile and Gly26Ser polymorphism with lipoprotein in patients with coronary heart disease

目的:探讨冠心病(CHD)患者内皮脂肪酶基因(LIPG)Thr111Ile和Gly26Ser的多态性与脂蛋白代谢的关系。方法:438例冠状动脉造影患者,根据造影结果分为CHD组(242例)和对照组(196例)。应用酶法测定患者的总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)水平。同时应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RELP)核苷酸分型技术检测Thr111Ile和Gly26Ser多态性,进行统计学分析。结果:Thr111Ile在本研究人群中的基因频率分布为CC76.7%、CT23.3%、TT0.0%,等位基因频率为C88.3%、T11.7%。CT组HDL-c水平高于CC组(P<0.05),经多因素逻辑回归分析,CT基因仍与高HDL-c水平呈显著相关(P<0.05),CHD与Thr111Ile多态性无显著相关(P>0.05)。未发现Gly26Ser基因变异。结论:中国汉族人群中存在LIPG基因的Thr111Ile多态性。Thr111Ile多态性中的CT基因致HDL-c水平升高,但未发现其与CHD直接相关。本研究未发现Gly26Ser多态性。

AIM： To investigate the association of endothelial lipase gene （LIPG） Thr111Ile and Gly26Ser polymorphism with lipoprotein in patients with coronary heart disease （CHD） in Chinese. METHODS： 438 patients were classified as 242 CHD group and 196 controls group by selective coronary angiography. Plasma level of lipoprotein was determined and the Thr111Ile and Gly26Ser polymorphism was screened by PCR - RELP. RESULTS： The frequencies of Thr111Ile genotype in Chinese were CC 76. 7%, CT 23.3%, TT 0. 0%. The frequencies of allele were C 88. 3%, T 11.7%. The plasma level of HDL -c in CT group was significantly higher than that in CC group （P 〈0. 05） on logistic regression analysis. However, logistic regression analysis revealed that there was no significant difference between CHD group and control group for Thr111Ile polymorphism （ P 〉 0. 05）. No Gly26Ser mutation was observed in this study. CONCLUSION： The polymorphism of Thr111Ile is present in patients with CHD in Chinese, and T allele is related to high HDL - c level. There is no significant association between the polymorphism of Thr111Ile and CHD. The Gly26Ser mutation has not found in this study.