环境压力对中国树鼩生殖生理影响的实验研究

Effect of environment stress on reproductive physiology in Chinese tree shrew

目的:探讨环境压力对中国树鼩生殖生理的影响。方法:中国树鼩30只,随机分为3组,1组为对照组10只,2组8只,3组12只。实验组采用缩小饲育环境、增大同种性别动物密度的方法,用放免仪测定性激素水平,用光镜观察睾丸的组织结构。结果:经分笼饲养21d测定,2、3组动物血清睾酮(T)比1组分别低49.4%和80.3%(P<0.01);雌三醇(E2)水平各组间差异无显著;光镜观察发现,2组动物睾丸组织曲细精管在高倍镜下可见精原细胞与支持细胞网架;3组仅见少数几个精原细胞。结论:环境压力能抑制中国树鼩睾酮形成,损伤睾丸组织结构。

AIM： To study the effect of environment stress on reproductive physiology in Chinese tree shrew. METHODS ： 30 animals were divided at random into three groups. Group 1 ： 10 of 30 animals served as control ; group 2 and 3 the 8 and 12 of 30 animals served as experiment and housed in small cage. The levels of the testosterone（T） and estradiol （ E2 ） were determined by the method of radi y and section preparation of testis was observed by light microscope after living in the reduced scope for 21days. RESULTS： The result showed that the levels of the testosterone in group 2 and 3 were decreased by 49.4% and 80. 3%, respectively, compared to that in groupl. The convoluted tubules of testis in group 2 and 3 were damaged and only a few spermatogonia was doserved. CONCLUSION： Social environment stress has significantly inhibitory effect on testosterone formation and destroy the testis structure of Chinese tree shrew.