实验性自身免疫性脑脊髓炎脑干听觉通路损害的研究

Study on the damage of auditory path of the experimental autoimmune encephalomyelitis

目的:探讨实验性自身免疫性脑脊髓炎(EAE)大鼠听觉通路损害的情况,为临床研究提供实验依据。方法:以豚鼠全脊髓匀浆(GPSCH)为抗原免疫Wistar大鼠建立EAE的动物模型,采用诱发电位仪检测实验组与对照组的脑干听觉诱发电位,通过脑干及耳蜗神经核HE染色及Weil髓鞘染色从解剖病理的角度证实脑干及耳蜗神经核损害。通过大鼠行为学的变化及光镜下脑和脊髓的病理改变来确定EAE的成功。结果:EAE大鼠的BAEP改变:左耳:Ⅳ、Ⅴ波潜伏期及Ⅰ-Ⅴ波峰间伏期IPL长于对照组(P<0.05),右耳:Ⅰ、Ⅱ波潜伏期及Ⅰ-ⅢⅢ-ⅤⅠ-Ⅴ波峰间伏期IPL长于对照组(P<0.05);而Ⅲ、Ⅳ、Ⅴ等波的潜伏期显著长于对照组(P<0.01)。病理图像显示脑干耳蜗神经核HE染色有大量淋巴细胞浸润,呈袖套样改变,髓鞘染色显示弥漫性脱髓鞘病变。结论:Wistar大鼠实验性自身免疫性脑脊髓炎(EAE)听神经通路有损害。

AIM： To explore if there is lesion in the auditory path in the brainstem of the Wistar rat with experimental autoimmune encephalomyelitis（EAE）. METHODS： EAE was induced by guinea pig spinal cord homogenate （ GPSCH）, then the evoked potential in both EAE and control groups was examined. The HE staining and myelin staining of the auditory nucleus were conducted in order to corroborate the damage of the auditory path in the brainstem. Through studying the pathological changes using light microscopy and behavior changes of rats, we defined that the model was successful. RESULTS： The latency period of auditory evoked potentials of EAE rats was much longer than that in control group. The pathological pictures manifested that auditory nucleus were invaded by a great number of lymphocytes, demyelinations were discovered in it. CONCLUSION： The auditory path in the brainstem of the Wistar rat with EAE is suffered from inflammation and demyelination.