腹膜间皮细胞在小鼠子宫内膜细胞刺激腹腔微环境中作用

Function of peritoneal mesothelial cells on the microenvironment by administration of endometrial cells

目的:探讨腹腔微环境受子宫内膜细胞刺激改变中腹膜间皮细胞的作用及其对子宫内膜异位症发病机制的意义。方法:注射小鼠子宫内膜上皮和间质细胞入小鼠腹腔,酶联免疫吸附法(ELISA)检测注射后4、24和72h时点腹腔冲洗液细胞因子MCP-1/JE、IL-1α和IL-6蛋白表达,同步逆转录-聚合酶链式反应技术(Real-Time RT-PCR)检测腹膜组织(主要含腹膜间皮细胞)和腹腔巨噬细胞的细胞因子MCP-1/JE、IL-1α和IL-6mRNA表达。结果:子宫内膜细胞刺激腹腔细胞因子蛋白含量迅速一过性升高,4h时点为表达高峰,腹膜细胞因子基因表达与腹腔液蛋白表达同步,腹腔巨噬细胞基因表达高峰滞后于腹腔液蛋白表达。子宫内膜上皮细胞刺激腹腔炎症反应作用强于间质细胞。结论:子宫内膜细胞刺激腹腔发生非特异性炎症反应,腹膜间皮细胞可能是其细胞因子效应的主要来源,提示腹膜在子宫内膜异位症中除作为病灶依附体外,还可能在腹腔微环境无菌性炎症反应中起重要作用。

AIM： To determine the function of peritoneal mesothelial cells on the inflammatory ment by administration of endometrial cells, and further define the pathogenesis of endometriosis. METHODS ： Homogenous mouse endometrial epithelial and stromal cells were injected into the peritoneal cavities of Swiss Webster mice. After 4, 24, and 72 h, a number of endpoints evaluated ： protein concentrations of cytokine MCP - 1, IL - 1α, IL - 6 in peritoneal lavage and gene expressions of MCP - 1, IL - 1α, IL - 6 in peritoneal mesothdial cells and maerophages. RESULTS： The intraperitoneal administration of endometrial cells increased the protein expressions of cytokines in the peritoneal lavage of the recipient mice, which increased at 4 - hour points and subsequently decreased with time. Gene expressions of cytokines in peritoneal mesothelial cells paralleled with the protein quantities in peritoneal lavage. The peak time of gene expression of cytokines in peritoneal maerophages was at the 24 - hour point. The endometrial epithelial cells stimulated stronger inflammatory responses in the peritoneal cavity than the endometrial stromal cells. CONCLUSION： The recipient mice have a non - specific inflammatory response to the presence of endometrial cells in the peritoneal cavity. Mesothelial cells may be the targets of early inflammatory stress initiated in the presence of endometrial cells.