正交试验法优选毛泡桐中乌索酸提取工艺条件

Optimum Extraction of Ursolic Acid in Paulownia tomentosa by Orthogonal Test

采用正交设计L9(34)方法,考察乙醇浓度(A)、超声时间(B)、超声功率(C)、料液比(D)对乌索酸提取率的影响,用高效液相色谱法测定含量,并与常规提取法进行对比,确定了毛泡桐中乌索酸的最佳超声提取工艺条件。所考察的因素对毛泡桐中乌索酸提取的影响按各因素作用主次顺序为:乙醇浓度>料液比>超声时间>超声功率;乌索酸超声提取的最佳条件为:A3B3C2D1,即毛泡桐叶粉末用6倍量体积分数95%乙醇超声提取2 h,超声功率为200 w。与常规的提取方法相比,超声提取具有提取时间短、操作简单、提取率高、无需加热等优点。优选的工艺条件稳定,操作简便,方法可行,可用于毛泡桐中乌索酸的提取。

According to the orthogonal design L9 （3^4）, the optimum ultrasonic extraction technology was sought through experimental investigation, and was compared with the traditional extraction technology. The content of ursolic acid was determined by RP- HPLC and the ultrasonic extraction for ursolic acid from Paulownia tomentosa was optimized. The order of factors to affect the ursolic acid extraction was A3B3C2D1. Optimum conditions of extraction by ultrasonic were determined as follows： ratio of material to liquor 1 ： 6, 95% ethanol, duration 2 h, efficiency 200 W. Compared with the traditional, ultrasonic method could save time, was easy to operate, could improve extraction rate and need no heating.