有机镓治疗维甲酸诱导骨质疏松模型大鼠的实验研究

Organic gallium improves tretinoin-induced osteoporosis in rats

目的对比研究有机镓和无机镓对维甲酸致大鼠骨质疏松模型骨代谢的影响及治疗作用。方法用维甲酸按85mg/(kg·d)灌胃15d,建立大鼠骨质疏松模型,经有机镓、氯化镓灌胃给药治疗4周后,应用生化、病理等方法研究其对骨代谢相关指标的影响,并比较其治疗效果。结果维甲酸可引起大鼠骨结构、骨钙及其它元素含量的改变,血清抗酒石酸酸性磷酸酶(TRAP)、碱性磷酸酶(AKP)明显上升等改变。应用有机镓、氯化镓治疗后,可改善骨密度及骨形态计量学参数,增加股骨和牙齿中的镓、钙、磷含量,降低TRAP和AKP活性,其中以有机镓效果最明显,且用量最少。结论在动物实验中,镓盐能通过降低骨转换率而改善骨质疏松,且有机镓较无机镓更安全、有效。

Objective To investigate the effect of organic gallium and gallium chloride on bone metabolism and their therapeutic effect against tretinoin-induced osteoporosis in rats. Methods Rat models of osteoporosis was established with intragastric administration oftretinoin at the daily dose of 85mg/kg for 15 days and randomized into control, organic gallium and gallium chloride groups. After administration of the corresponding treatments （none for the control group） for 4 weeks, the changes of the indices for osteoporosis were evaluated through biochemical and pathological approaches. Results Tretinoin induced obvious changes in bone structure and contents of bone calcium and other elements, causing also significantly increased tartrate-resistant acid phosphatase （TRAP） and alkaline phosphatase （AKP）, which suggested the development of osteoporosis. Administration of organic gallium and gallium chloride treatments increased the bone density, bone cortex thickness and the percentage of bone trabecula, and Ga, Ca, P contents in the femur and teeth, but lowered the activity of TRAP and AKP, suggesting decreased bone conversion rate. Compared with gallium chloride, organic gallium required smaller dose with better safety to produce better therapeutic effect. Conclusion Organic gallium can be safe and effective for treatment of tretinoin-induced osteoporosis in rats.